Product nameAnti-LYAR antibody [EPR14352-9]
See all LYAR primary antibodies
DescriptionRabbit monoclonal [EPR14352-9] to LYAR
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human LYAR aa 150-350. The exact sequence is proprietary.
Database link: Q9NX58
- Ramos, Jurkat, A431 and K562 cell lysates; K562 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab182138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).|
Sequence similaritiesContains 2 C2HC-type zinc fingers.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Cell growth regulating nucleolar protein antibody
- Cell growth-regulating nucleolar protein antibody
- Likely ortholog of mouse Ly1 antibody reactive clone antibody
All lanes : Anti-LYAR antibody [EPR14352-9] (ab182138) at 1/20000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab182138 at 1/60 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling LYAR with ab182138 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI (blue).
ab182138 has not yet been referenced specifically in any publications.