Product nameAnti-LYK5 antibody
See all LYK5 primary antibodies
DescriptionRabbit polyclonal to LYK5
SpecificityThe amino acid sequence used as immunogen is 100% homologous in isoforms 1, 2, 3 and 4 of Human LYK5.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
Synthetic peptide consisting of a portion of amino acids 300-350 of Human LYK5 (isoform 1).
- A549 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab47103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 48 kDa).|
RelevanceMembers of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
Cellular localizationCytoplasmic and Nuclear
- Protein kinase LYK5 antibody
- Serologically defined breast cancer antigen NY BR 96 antibody
- STE20 related adapter protein antibody
All lanes : Anti-LYK5 antibody (ab47103) at 1 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate with immunizing peptide
Predicted band size: 48 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
ab47103 has not yet been referenced specifically in any publications.