Anti-Lymphotoxin Alpha + Beta antibody [MM0460-12B4] (ab89566)

Overview

  • Product name

    Anti-Lymphotoxin Alpha + Beta antibody [MM0460-12B4]
  • Description

    Mouse monoclonal [MM0460-12B4] to Lymphotoxin Alpha + Beta
  • Host species

    Mouse
  • Specificity

    ab89566 detects Lymphotoxin Alpha and Beta.
  • Tested applications

    Suitable for: Flow Cyt, Neutralisingmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human Lymphotoxin Alpha + Beta.

Properties

Applications

Our Abpromise guarantee covers the use of ab89566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Neutralising Use at an assay dependent concentration.

Target

  • Relevance

    Lymphotoxin Alpha, which is a member of the TNF family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with Lymphotoxin Beta which anchors Lymphotoxin Alpha to the cell surface. This protein is also involved in the formation of secondary lymphoid organs during development, mediates a variety of inflammatory, immunostimulatory, and antiviral responses, and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis. Lymphotoxin Beta is a type II membrane protein of the TNF family. It anchors Lymphotoxin Alpha to the cell surface through heterotrimer formation. The predominant form on the lymphocyte surface is the Lymphotoxin Alpha 1/Beta 2 complex (e.g. 1 molecule alpha/2 molecules beta), which is the primary ligand for the Lymphotoxin Beta receptor. The minor complex is Lymphotoxin Alpha 2/Beta 1. Lymphotoxin Beta is an inducer of the inflammatory response system and involved in normal development of lymphoid tissue. Lymphotoxin Beta isoform b is unable to complex with Lymphotoxin Alpha suggesting a function for Lymphotoxin Beta which is independent of Lymphotoxin Alpha. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Cellular localization

    Lymphotoxin Alpha: Cell Membrane and Secreted. Note: The homotrimer is secreted. The heterotrimer is membrane associated. Lymphotoxin Beta: Membrane; Single pass type II membrane protein.
  • Database links

  • Alternative names

    • LT alpha antibody
    • LT antibody
    • LT beta antibody
    • Lymphotoxin alpha (TNF superfamily member 1) antibody
    • Lymphotoxin beta (TNF superfamily member 3) antibody
    • p33 antibody
    • TNF beta antibody
    • TNF C antibody
    • TNFB antibody
    • TNFSF1 antibody
    • TNFSF3 antibody
    • Tumor necrosis factor beta antibody
    • Tumor necrosis factor C antibody
    • Tumor necrosis factor ligand superfamily member 1 antibody
    • Tumor necrosis factor ligand superfamily member 3 antibody
    see all

Images

  • ab89566 staining Lymphotoxin Alpha + Beta in PBMC, T cell progenitors by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 1X PBS) for 15 minutes at 4°C. A Alexa Fluor® -conjugated donkey anti-mouse IgG (1/20) was used as the secondary antibody.
    Gating Strategy: CD7+ cells.

    See Abreview

References

ab89566 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

DISCOUNT CODE: XXXXXXXXXXX
Expiration date: 21-09-2012

I am very pleased to hear you would like to accept our offer and test ab89566 in [IHC-P]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [IHC-P] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Flow Cytometry
Fixation
No fixation
Sample
Human Cell (PBMC, T cells progenitors)
Specification
PBMC, T cells progenitors
Gating Strategy
CD7+ cells
Permeabilization
No

Dr. Elodie Elkaim

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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