Anti-Lyn antibody (ab227170)
Key features and details
- Rabbit polyclonal to Lyn
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Lyn antibody
See all Lyn primary antibodies -
Description
Rabbit polyclonal to Lyn -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow -
Immunogen
Recombinant fragment within Human Lyn (internal sequence). The exact sequence is proprietary.
Database link: P07948 -
Positive control
- WB: THP-1 whole cell lysate. IP: K562 whole cell extract. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 59 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 59 kDa. |
Target
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Function
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
Tissue specificity
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Domain
The protein kinase domain plays an important role in its localization in the cell membrane. -
Post-translational
modificationsUbiquitinated. Ubiquitination is SH3-dependent. -
Cellular localization
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
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Database links
- Entrez Gene: 534996 Cow
- Entrez Gene: 4067 Human
- Entrez Gene: 17096 Mouse
- Entrez Gene: 81515 Rat
- Entrez Gene: 100302103 Sheep
- Omim: 165120 Human
- SwissProt: P07948 Human
- SwissProt: P25911 Mouse
see all -
Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- AA407514 antibody
- EC 2.7.10.2 antibody
- FLJ26625 antibody
see all
Images
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Anti-Lyn antibody (ab227170) at 1/1000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 59 kDa7.5 % SDS-PAGE gel.
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PFA-fixed A431 (human epidermoid carcinoma cell line) cells stained for Lyn (green) using ab227170 at 1/500 dilution in ICC/IF.
Counterstain: Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (red) at 1/2000 dilution.
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Lyn was immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract with 4 µg of ab227170. Western blot was performed from the immunoprecipitate using ab227170 at 1/500 dilution. An Anti-Rabbit IgG was used as secondary antibody.
Lane 1: K562 whole cell lysate 40 µg (Input).
Lane 2: Rabbit IgG instead of ab227170 in K562 whole cell lysate.
Lane 3: ab227170 IP in K562 whole cell lysate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227170 has not yet been referenced specifically in any publications.