Overview

  • Product name

  • Description

    Rabbit polyclonal to Lyn
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow
  • Immunogen

    Recombinant fragment within Human Lyn (internal sequence). The exact sequence is proprietary.
    Database link: P07948

  • Positive control

    • WB: THP-1 whole cell lysate. IP: K562 whole cell extract. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.0
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1% BSA, 20% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.

Target

  • Function

    Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
  • Tissue specificity

    Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Domain

    The protein kinase domain plays an important role in its localization in the cell membrane.
  • Post-translational
    modifications

    Ubiquitinated. Ubiquitination is SH3-dependent.
  • Cellular localization

    Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
  • Information by UniProt
  • Database links

  • Form

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names

    • AA407514 antibody
    • EC 2.7.10.2 antibody
    • FLJ26625 antibody
    • Hck 2 antibody
    • JTK 8 antibody
    • JTK8 antibody
    • Lck/Yes related novel protein tyrosine kinase antibody
    • LYN antibody
    • LYN proto oncogene, Src family tyrosine kinase antibody
    • LYN_HUMAN antibody
    • ONCOGENE LYN antibody
    • p53Lyn antibody
    • p56Lyn antibody
    • Tyrosine protein kinase LYN antibody
    • Tyrosine-protein kinase Lyn antibody
    • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
    • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
    see all

Images

  • Anti-Lyn antibody (ab227170) at 1/1000 dilution + THP-1 (human monocytic leukemia cell line) whole cell lysate at 30 µg

    Predicted band size: 59 kDa



    7.5 % SDS-PAGE gel.

     

  • PFA-fixed A431 (human epidermoid carcinoma cell line) cells stained for Lyn (green) using ab227170 at 1/500 dilution in ICC/IF.

    Counterstain: Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (red) at 1/2000 dilution.

  • Lyn was immunoprecipitated from K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract with 4 µg of ab227170. Western blot was performed from the immunoprecipitate using ab227170 at 1/500 dilution. An Anti-Rabbit IgG was used as secondary antibody.

    Lane 1: K562 whole cell lysate 40 µg (Input). 

    Lane 2: Rabbit IgG instead of ab227170 in K562 whole cell lysate. 

    Lane 3: ab227170 IP in K562 whole cell lysate.

References

ab227170 has not yet been referenced specifically in any publications.

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