Anti-Lyn antibody [LYN-01] (ab1890)
Key features and details
- Mouse monoclonal [LYN-01] to Lyn
- Suitable for: Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Lyn antibody [LYN-01]
See all Lyn primary antibodies -
Description
Mouse monoclonal [LYN-01] to Lyn -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Lyn aa 1-250.
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General notes
Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Primary antibody notes
Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI). -
Clonality
Monoclonal -
Clone number
LYN-01 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB | (3) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa. |
Target
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Function
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
Tissue specificity
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Domain
The protein kinase domain plays an important role in its localization in the cell membrane. -
Post-translational
modificationsUbiquitinated. Ubiquitination is SH3-dependent. -
Cellular localization
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
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Database links
- Entrez Gene: 4067 Human
- Entrez Gene: 81515 Rat
- Omim: 165120 Human
- SwissProt: P07948 Human
- SwissProt: Q07014 Rat
- Unigene: 491767 Human
- Unigene: 4338 Rat
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Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- AA407514 antibody
- EC 2.7.10.2 antibody
- FLJ26625 antibody
see all
Images
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All lanes : Anti-Lyn antibody [LYN-01] (ab1890) at 1 µg/ml
Lane 1 : Negative control
Lane 2 : Rat basophilic leukemia cell line
Lane 3 : JURKAT human peripheral blood T cell leukemia cell line
Lane 4 : A-431 human epidermoid carcinoma cell line
Lane 5 : U-937 human histiocytic lymphoma cell line
Predicted band size: 58 kDaNon reducing conditions with 60 minutes exposure time in a 12% gel
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Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + Recombinant human Lyn protein (ab60306) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 4 minutes -
Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 50 µg
Secondary
HRP-conjugated anti-mouse at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
Overlay histogram showing Ramos (Human Burkitt's lymphoma cell line) cells stained with ab1890 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1890, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab1890 has been referenced in 18 publications.
- Sangha R et al. Novel, First-in-Human, Oral PCLX-001 Treatment in a Patient with Relapsed Diffuse Large B-Cell Lymphoma. Curr Oncol 29:1939-1946 (2022). PubMed: 35323358
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Brian BF et al. Unique-region phosphorylation targets LynA for rapid degradation, tuning its expression and signaling in myeloid cells. Elife 8:N/A (2019). PubMed: 31282857
- Gavali S et al. Estrogen enhances human osteoblast survival and function via promotion of autophagy. Biochim Biophys Acta Mol Cell Res 1866:1498-1507 (2019). PubMed: 31255720
- Lin J et al. Characterizing the molecular mechanisms of acquired temozolomide resistance in the U251 glioblastoma cell line by protein microarray. Oncol Rep 39:2333-2341 (2018). PubMed: 29565460