Recombinant Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP504Y] to Lyn (phospho Y507)
- Suitable for: Dot blot, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Lyn (phospho Y507) antibody [EP504Y]
See all Lyn primary antibodies -
Description
Rabbit monoclonal [EP504Y] to Lyn (phospho Y507) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Lyn (phospho Y507). The exact sequence is proprietary.
Database link: P07948 -
Positive control
- WB: HeLa (untreated and treated with pervanadate) and Raji treated with pervanadate whole cell lysates. IP: SH-SY5Y treated with pervandate whole cell lysate.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP504Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Dot blot |
1/1000.
|
|
WB |
1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa).
|
|
IP |
1/80.
|
Notes |
---|
Dot blot
1/1000. |
WB
1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa). |
IP
1/80. |
Target
-
Function
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
Tissue specificity
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Domain
The protein kinase domain plays an important role in its localization in the cell membrane. -
Post-translational
modificationsUbiquitinated. Ubiquitination is SH3-dependent. -
Cellular localization
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
-
Database links
- Entrez Gene: 4067 Human
- Entrez Gene: 17096 Mouse
- Entrez Gene: 81515 Rat
- Omim: 165120 Human
- SwissProt: P07948 Human
- SwissProt: P25911 Mouse
- SwissProt: Q07014 Rat
- Unigene: 491767 Human
see all -
Form
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
Alternative names
- AA407514 antibody
- EC 2.7.10.2 antibody
- FLJ26625 antibody
see all
Images
-
All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : Raji treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Raji treated with 1mM pervanadate for 30 min whole cell lysate, the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDaBlocking and dilution buffer: 5% NFDM/TBST.
-
Purified ab33914 at 1/80 dilution (2µg) immunoprecipitating Lyn in SH-SY5Y treated with pervandate whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) treated with pervandate whole cell lysate 10µg
Lane 2 (+): ab33914 + SH-SY5Y treated with pervandate whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab256224 in SH-SY5Y treated with pervandate whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 61 kDa -
Dot blot analysis of Lyn (phospho Y507) phospho peptide (Lane 1) and Lyn non-phospho peptide (Lane 2) labelling Lyn (phospho Y507) with ab33914 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.
Blocking and dilution buffer: 5% NFDM /TBST.
-
All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilution
Lane 1 : 10ug Non treated HeLa cell lysate
Lane 2 : 10ug HeLa cell lysate treated with Pervanadate
Secondary
All lanes : Goat anti-rabbit HRP labeled.
Predicted band size: 61 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (11)
ab33914 has been referenced in 11 publications.
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Xu Y et al. MicroRNA-218-5p inhibits cell growth and metastasis in cervical cancer via LYN/NF-?B signaling pathway. Cancer Cell Int 18:198 (2018). PubMed: 30524205
- Gavali S et al. LYN, a key mediator in estrogen-dependent suppression of osteoclast differentiation, survival, and function. Biochim Biophys Acta Mol Basis Dis 1865:547-557 (2018). PubMed: 30579930
- Tondera C et al. Insights into binding of S100 proteins to scavenger receptors: class B scavenger receptor CD36 binds S100A12 with high affinity. Amino Acids 49:183-191 (2017). PubMed: 27734162
- Sharma N et al. SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. J Immunol 192:4859-66 (2014). PubMed: 24733849