Recombinant
RabMAb

Recombinant Anti-LYPD3 antibody [SP208] (ab183357)

Overview

  • Product name

    Anti-LYPD3 antibody [SP208]
    See all LYPD3 primary antibodies
  • Description

    Rabbit monoclonal [SP208] to LYPD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LYPD3 aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: O95274

  • Positive control

    • IHC-P: Human skin, esophagus, tonsil, bladder, cervix, breast ductal carcinoma, skin squamous cell carcinoma, and cervical squamous cell carcinoma tissue.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP208
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.
  • Tissue specificity

    Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers.
  • Sequence similarities

    Contains 2 UPAR/Ly6 domains.
  • Post-translational
    modifications

    N-glycosylated and O-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310061G07Rik antibody
    • C4.4A antibody
    • GPI-anchored metastasis-associated protein C4.4A homolog antibody
    • GPI-anchored metastasis-associated protein homolog antibody
    • Ly6/PLAUR domain-containing protein 3 antibody
    • Lypd3 antibody
    • LYPD3_HUMAN antibody
    • Matrigel-induced gene C4 protein antibody
    • MIG-C4 antibody
    • SHINC3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervix carcinoma tissue sections labeling LYPD3 with ab183357 at 1/100 dilution (4.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling LYPD3 with ab183357 at 1/100 dilution (4.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling LYPD3 with ab183357 at 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human esophagus tissue labeling LYPD3 with ab183357 at 1/100 dilution.

     

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LYPD3 with ab183357 at 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder tissue labeling LYPD3 with ab183357 at 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervix tissue labeling LYPD3 with ab183357 at 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast ductal cell carcinoma tissue labeling LYPD3 with ab183357 at 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin squamous cell carcinoma tissue labeling LYPD3 with ab183357 at 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical squamous cell carcinoma tissue labeling LYPD3 with ab183357 at 1/100 dilution.

     

References

ab183357 has not yet been referenced specifically in any publications.

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