Recombinant Anti-LYPD3 antibody [SP250] - BSA and Azide free (ab271958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP250] to LYPD3 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LYPD3 antibody [SP250] - BSA and Azide free
See all LYPD3 primary antibodies -
Description
Rabbit monoclonal [SP250] to LYPD3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skin tissue. Flow Cytometry: BxPC-3 cells.
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General notes
ab271958 is the carrier-free version of ab192845.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
SP250 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-LYPD3 antibody [SP250] - N-terminal (ab192845)
- Alexa Fluor® 488 Anti-LYPD3 antibody [SP250] - N-terminal (ab309938)
- Alexa Fluor® 647 Anti-LYPD3 antibody [SP250] - N-terminal (ab310305)
- Alexa Fluor® 594 Anti-LYPD3 antibody [SP250] - N-terminal (ab310768)
- Alexa Fluor® 555 Anti-LYPD3 antibody [SP250] - N-terminal (ab312295)
- Alexa Fluor® 568 Anti-LYPD3 antibody [SP250] - N-terminal (ab312790)
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature
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Flow Cyt |
Use at an assay dependent concentration.
Incubate with primary antibody for 30 minutes at 4°C.
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Notes |
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IHC-P
Use at an assay dependent concentration. Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature
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Flow Cyt
Use at an assay dependent concentration. Incubate with primary antibody for 30 minutes at 4°C.
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Target
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Function
Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. -
Tissue specificity
Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated and O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27076 Human
- Entrez Gene: 72434 Mouse
- Entrez Gene: 60378 Rat
- Omim: 609484 Human
- SwissProt: O95274 Human
- SwissProt: Q91YK8 Mouse
- SwissProt: O55162 Rat
- Unigene: 631594 Human
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Alternative names
- 2310061G07Rik antibody
- C4.4A antibody
- GPI-anchored metastasis-associated protein C4.4A homolog antibody
see all
Images
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Flow cytometric analysis of BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling LYPD3 with ab192845 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192845).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab271958 has not yet been referenced specifically in any publications.