Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP250] to LYPD3 - N-terminal
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-LYPD3 antibody [SP250] - N-terminal
See all LYPD3 primary antibodies
DescriptionRabbit monoclonal [SP250] to LYPD3 - N-terminal
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human LYPD3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O95274
- IHC-P: Human skin tissue. Flow Cytometry: BxPC-3 cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by Protein A/G.
Our Abpromise guarantee covers the use of ab192845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Incubate with primary antibody for 10 minutes at room temperature
Incubate with primary antibody for 30 minutes at 4°C.
FunctionSupports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.
Tissue specificityExpressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers.
Sequence similaritiesContains 2 UPAR/Ly6 domains.
modificationsN-glycosylated and O-glycosylated.
Cellular localizationCell membrane.
- Information by UniProt
- 2310061G07Rik antibody
- C4.4A antibody
- GPI-anchored metastasis-associated protein C4.4A homolog antibody
Formalin-fixed, paraffin-embedded human skin tissue stained for LYPD3 using ab192845 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling LYPD3 with ab192845 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab192845 has not yet been referenced specifically in any publications.