Recombinant
RabMAb

Recombinant Anti-LYPD3 antibody [SP250] - N-terminal (ab192845)

Overview

  • Product name

    Anti-LYPD3 antibody [SP250] - N-terminal
    See all LYPD3 primary antibodies
  • Description

    Rabbit monoclonal [SP250] to LYPD3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human LYPD3 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O95274

  • Positive control

    • IHC-P: Human skin tissue. Flow Cytometry: BxPC-3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP250
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 10 minutes at room temperature

Flow Cyt 1/100.

Incubate with primary antibody for 30 minutes at 4°C.

Target

  • Function

    Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.
  • Tissue specificity

    Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers.
  • Sequence similarities

    Contains 2 UPAR/Ly6 domains.
  • Post-translational
    modifications

    N-glycosylated and O-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2310061G07Rik antibody
    • C4.4A antibody
    • GPI-anchored metastasis-associated protein C4.4A homolog antibody
    • GPI-anchored metastasis-associated protein homolog antibody
    • Ly6/PLAUR domain-containing protein 3 antibody
    • Lypd3 antibody
    • LYPD3_HUMAN antibody
    • Matrigel-induced gene C4 protein antibody
    • MIG-C4 antibody
    • SHINC3 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human skin tissue stained for LYPD3 using ab192845 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling LYPD3 with ab192845 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).

References

ab192845 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (Thymic epithelial cells)
Permeabilization
No
Gating Strategy
CD45- EpCAM+ MHCII lo L1cam lo Itgb4 lo - gating on post Aire TECs
Specification
Thymic epithelial cells

Abcam user community

Verified customer

Submitted Oct 11 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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