Recombinant Anti-LYPD3 antibody [SP250] - N-terminal (ab192845)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP250] to LYPD3 - N-terminal
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LYPD3 antibody [SP250] - N-terminal
See all LYPD3 primary antibodies -
Description
Rabbit monoclonal [SP250] to LYPD3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skin tissue. Flow Cytometry: BxPC-3 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
SP250 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature |
|
Flow Cyt | (1) |
1/100.
Incubate with primary antibody for 30 minutes at 4°C. |
Notes |
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IHC-P
1/100. Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature |
Flow Cyt
1/100. Incubate with primary antibody for 30 minutes at 4°C. |
Target
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Function
Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. -
Tissue specificity
Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. -
Sequence similarities
Contains 2 UPAR/Ly6 domains. -
Post-translational
modificationsN-glycosylated and O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27076 Human
- Entrez Gene: 72434 Mouse
- Entrez Gene: 60378 Rat
- Omim: 609484 Human
- SwissProt: O95274 Human
- SwissProt: Q91YK8 Mouse
- SwissProt: O55162 Rat
- Unigene: 631594 Human
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Alternative names
- 2310061G07Rik antibody
- C4.4A antibody
- GPI-anchored metastasis-associated protein C4.4A homolog antibody
see all
Images
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Formalin-fixed, paraffin-embedded human skin tissue stained for LYPD3 using ab192845 at 1/100 dilution in immunohistochemical analysis.
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Flow cytometric analysis of BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling LYPD3 with ab192845 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab192845 has not yet been referenced specifically in any publications.