Product nameAnti-LYPLAL1 antibody
See all LYPLAL1 primary antibodies
DescriptionGoat polyclonal to LYPLAL1
Tested applicationsSuitable for: WB, ELISA, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Orangutan
- Human Adipose and Heart lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77640 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the AB hydrolase 2 family.
- Information by UniProt
- BC027340 antibody
- FLJ99730 antibody
- KIAA1238 antibody
Anti-LYPLAL1 antibody (ab77640) at 0.3 µg/ml + Human adipose lysate in RIPA buffer at 35 µg
Predicted band size: 26 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Immunofluorescence analysis of mouse retina, retinal pigment epithelium and sclera in induced myopic eye (bottom left), fellow non-occluded eye (bottom right), and independent control eye (top right).
LYPLAL1 (green) was stained using ab77640 at 1/200 dilution. A fluorescein-conjugated rabbit anti-goat secondary antibody was used at 1/800 dilution. Nuclei were stained with DAPI (blue).
Negative control eye (top left) was treated with the secondary antibody alone and DAPI.
ab77640 has been referenced in 1 publication.
- Fan Q et al. Genetic variants on chromosome 1q41 influence ocular axial length and high myopia. PLoS Genet 8:e1002753 (2012). IHC-Fr ; Mouse . PubMed: 22685421