Overview

  • Product name
  • Description
    Rabbit polyclonal to LYRIC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human LYRIC.

    Read Abcam's proprietary immunogen policy (Peptide available as ab45337.)

  • Positive control
    • This antibody gave a positive signal in mouse and rat skeletal muscle tissue lysates, and in mouse heart tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skin cancer.

Properties

Applications

Our Abpromise guarantee covers the use of ab45338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
  • Tissue specificity
    Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
  • Cellular localization
    Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3D3 antibody
    • 3D3/LYRIC antibody
    • AEG 1 antibody
    • AEG-1 antibody
    • AEG1 antibody
    • Astrocyte elevated gene 1 antibody
    • Astrocyte elevated gene-1 protein antibody
    • LYRIC antibody
    • LYRIC/3D3 antibody
    • LYRIC_HUMAN antibody
    • Lysine rich CEACAM1 associated protein antibody
    • Lysine rich CEACAM1 co isolated protein antibody
    • Lysine-rich CEACAM1 co-isolated protein antibody
    • Metadherin antibody
    • Metastasis adhesion protein antibody
    • MTDH antibody
    • Protein LYRIC antibody
    see all

Images

  • All lanes : Anti-LYRIC antibody (ab45338) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : DU145 (Human Prostate carcinoma epithelial like cell line) Whole Cell lysate
    Lane 5 : PC3 whole cell lysate (ab3954)
    Lane 6 : U87 MG nuclear extract lysate (ab14903)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 130 kDa, 80 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands.

  • All lanes : Anti-LYRIC antibody (ab45338) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa


    Exposure time: 1 minute
  • IHC image of LYRIC staining in Human skin cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45338, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

  • ICC/IF image of ab45338 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45338, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells fixed in 100% methanol (10 min) cells.

References

This product has been referenced in:
  • Yu X  et al. AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway. Biomed Res Int 2018:3145689 (2018). Read more (PubMed: 29770329) »
  • Fang L  et al. MicroRNA-625-3p promotes the proliferation, migration and invasion of thyroid cancer cells by up-regulating astrocyte elevated gene 1. Biomed Pharmacother 102:203-211 (2018). WB ; Human . Read more (PubMed: 29558717) »
See all 8 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

DISCOUNT CODE: XXXXXX
Expiration date: 22-02-2013
Value: £234

I am very pleased to hear you would like to accept our offer and test ab45338 in Flow Cytometry. This code will give you £234 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting us.

The concentration of this product is lot dependant. Current lot is at conc.1mg/ml.

This product has not been tested in Flow Cytometry yet. If you are interested in testing this product then I would encourage you to avail the 100% Abtrial testing discount programme. Please click the link below for more information;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (meiotic egg extract)
Specification
meiotic egg extract
Fixative
pre-fixation in formaldehyde and Methanol fixation after extract spindown onto coverslips
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 03 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
25 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 HS578T PC3)
Loading amount
20 µg
Specification
MCF7 HS578T PC3
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Antibody Solutions

Verified customer

Submitted Dec 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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