Overview

  • Product name

  • Description

    Rabbit polyclonal to LYRIC/AEG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human LYRIC/AEG1 (internal sequence). The exact sequence is proprietary.
    Database link: Q86UE4

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 cell lysate. ICC/IF: HeLa cells. IHC: Rat brain and intestine tissue.
  • General notes

     This product was previously labelled as LYRIC

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
  • Tissue specificity

    Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
  • Cellular localization

    Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3D3 antibody
    • 3D3/LYRIC antibody
    • AEG 1 antibody
    • AEG-1 antibody
    • AEG1 antibody
    • Astrocyte elevated gene 1 antibody
    • Astrocyte elevated gene-1 protein antibody
    • LYRIC antibody
    • LYRIC/3D3 antibody
    • LYRIC_HUMAN antibody
    • Lysine rich CEACAM1 associated protein antibody
    • Lysine rich CEACAM1 co isolated protein antibody
    • Lysine-rich CEACAM1 co-isolated protein antibody
    • Metadherin antibody
    • Metastasis adhesion protein antibody
    • MTDH antibody
    • Protein LYRIC antibody
    see all

Images

  • All lanes : Anti-LYRIC/AEG1 antibody (ab226808) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 64 kDa

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LYRIC/AEG1 (green) using ab226808 at 1/2500 dilution in ICC/IF.

  • Paraffin-embedded rat brain tissue stained for LYRIC/AEG1 using ab226808 at 1/2400 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat intestine tissue stained for LYRIC/AEG1 using ab226808 at 1/2400 dilution in immunohistochemical analysis.

References

ab226808 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab226808.
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