• Product name

    Anti-LYRIC/AEG1 antibody [EP4445]
    See all LYRIC/AEG1 primary antibodies
  • Description

    Rabbit monoclonal [EP4445] to LYRIC/AEG1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LYRIC/AEG1 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • HeLa, Jurkat, PC3, PC12 and NIH 3T3 cell lysates; Human brain and breast tissues; HeLa cells.
  • General notes

     This product was previously labelled as LYRIC


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
    • Tissue specificity

      Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
    • Cellular localization

      Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3D3 antibody
      • 3D3/LYRIC antibody
      • AEG 1 antibody
      • AEG-1 antibody
      • AEG1 antibody
      • Astrocyte elevated gene 1 antibody
      • Astrocyte elevated gene-1 protein antibody
      • LYRIC antibody
      • LYRIC/3D3 antibody
      • LYRIC_HUMAN antibody
      • Lysine rich CEACAM1 associated protein antibody
      • Lysine rich CEACAM1 co isolated protein antibody
      • Lysine-rich CEACAM1 co-isolated protein antibody
      • Metadherin antibody
      • Metastasis adhesion protein antibody
      • MTDH antibody
      • Protein LYRIC antibody
      see all


    • All lanes : Anti-LYRIC/AEG1 antibody [EP4445] (ab124789) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 64 kDa

    • ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

    • ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


    This product has been referenced in:

    • Wang F miR-384 targets metadherin gene to suppress growth, migration, and invasion of gastric cancer cells. J Int Med Res 47:926-935 (2019). Read more (PubMed: 30614349) »
    • Zhu YZ  et al. Metadherin overexpression in perihilar cholangiocarcinoma is associated with lymph node metastasis and poor prognosis. Oncol Lett 17:4514-4520 (2019). Read more (PubMed: 30988817) »
    See all 6 Publications for this product

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