Recombinant
RabMAb

Recombinant Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (ab239999)

Overview

  • Product name

    Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free
    See all LYRIC/AEG1 primary antibodies
  • Description

    Rabbit monoclonal [EP4445] to LYRIC/AEG1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human LYRIC/AEG1 aa 250-350. The exact sequence is proprietary.

  • General notes

    ab239999 is the carrier-free version of ab124789 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239999 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as LYRIC

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98 degrees C, allow to cool for 10-20 minutes)

 

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
    • Tissue specificity

      Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
    • Cellular localization

      Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3D3 antibody
      • 3D3/LYRIC antibody
      • AEG 1 antibody
      • AEG-1 antibody
      • AEG1 antibody
      • Astrocyte elevated gene 1 antibody
      • Astrocyte elevated gene-1 protein antibody
      • LYRIC antibody
      • LYRIC/3D3 antibody
      • LYRIC_HUMAN antibody
      • Lysine rich CEACAM1 associated protein antibody
      • Lysine rich CEACAM1 co isolated protein antibody
      • Lysine-rich CEACAM1 co-isolated protein antibody
      • Metadherin antibody
      • Metastasis adhesion protein antibody
      • MTDH antibody
      • Protein LYRIC antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124789).

    • ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124789).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124789).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab239999 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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