Key features and details
- Rabbit polyclonal to LYRM5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LYRM5 antibody
DescriptionRabbit polyclonal to LYRM5
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: Transfected HEK-293T whole cell lysates; IHC-P: Human stomach tissue; ICC: RH-30 whole cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 11 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the complex I LYR family.
- Information by UniProt
- LYR motif containing 5 antibody
- LYR motif-containing protein 5 antibody
- LYRM5 antibody
- LYRM5_HUMAN antibody
All lanes : Anti-LYRM5 antibody (ab126379) at 1/100 dilution
Lane 1 : Negative control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : LYRM5 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 11 kDa
Immunohistochemical analysis of human stomach tissue labeling LYRM5 in the cytoplasm of glandular cells with ab126379 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemical analysis of RH-30 (Human bone marrow metastasis cell line) whole cells labeling LYRM5 in the microtubule organizing center with ab126379 at 2 µg/ml.
ab126379 has not yet been referenced specifically in any publications.