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Anti-LYRM7 antibody (ab151089)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-LYRM7 antibody (ab151089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM7 antibody (ab151089)
  • Western blot - Anti-LYRM7 antibody (ab151089)

Key features and details

  • Rabbit polyclonal to LYRM7
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-LYRM7 antibody
  • Description

    Rabbit polyclonal to LYRM7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 12-104 of Human LYRM7 (UniProt: Q5U5X0).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human rectum tissue; RT 4, U 251 MG and Human liver lysates.
  • General notes

    The antibody solution should be gently mixed before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 12 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    The function of this protein remains unknown.
  • Cellular localization

    Mitochondrial
  • Database links

    • Entrez Gene: 90624 Human
    • Entrez Gene: 75530 Mouse
    • SwissProt: Q5U5X0 Human
    • SwissProt: Q9DA03 Mouse
    • Alternative names

      • C5orf31 antibody
      • FLJ20796 antibody
      • LYR motif containing 7 antibody
      • LYR motif containing protein 7 antibody
      • Lyrm7 homolog antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-LYRM7 antibody (ab151089)
      Immunocytochemistry/ Immunofluorescence - Anti-LYRM7 antibody (ab151089)
      Immunofluorescent staining of Human cell line HEK 293 shows positivity in cytoplasm and vesicles. Recommended concentration of ab151089 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM7 antibody (ab151089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM7 antibody (ab151089)
      Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling LYRM7 with ab151089 at 1/500 dilution.
    • Western blot - Anti-LYRM7 antibody (ab151089)
      Western blot - Anti-LYRM7 antibody (ab151089)
      All lanes : Anti-LYRM7 antibody (ab151089) at 1/500 dilution

      Lane 1 : RT 4 cell lysate
      Lane 2 : U 251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver tissue lysate
      Lane 5 : Huma tonsil tissue lysate

      Secondary

      Developed using the ECL technique.

      Predicted band size: 12 kDa

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab151089? Please let us know so that we can cite the reference in this datasheet.

    ab151089 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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