Anti-LYRM7 antibody (ab151089)
Key features and details
- Rabbit polyclonal to LYRM7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LYRM7 antibody -
Description
Rabbit polyclonal to LYRM7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment, corresponding to amino acids 12-104 of Human LYRM7 (UniProt: Q5U5X0).
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Positive control
- Human rectum tissue; RT 4, U 251 MG and Human liver lysates.
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General notes
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 12 kDa.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 12 kDa. |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
The function of this protein remains unknown. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 90624 Human
- Entrez Gene: 75530 Mouse
- SwissProt: Q5U5X0 Human
- SwissProt: Q9DA03 Mouse
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Alternative names
- C5orf31 antibody
- FLJ20796 antibody
- LYR motif containing 7 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-LYRM7 antibody (ab151089)Immunofluorescent staining of Human cell line HEK 293 shows positivity in cytoplasm and vesicles. Recommended concentration of ab151089 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM7 antibody (ab151089)Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling LYRM7 with ab151089 at 1/500 dilution. -
Western blot - Anti-LYRM7 antibody (ab151089)All lanes : Anti-LYRM7 antibody (ab151089) at 1/500 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver tissue lysate
Lane 5 : Huma tonsil tissue lysate
Secondary
Developed using the ECL technique.
Predicted band size: 12 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151089 has not yet been referenced specifically in any publications.