Product nameLysoLive™ Lysosomal Sulfatase Assay Kit
See all Sulfatase kits
The MarkerGene™LysoLive™ Lysosomal Sulfatase Assay Kit utilizes the sensitive substrate SulfGreen™ to monitor sulfatase activity in the lysosomes of live cells.
Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells. Lysosomes have been found to be involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis, and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinson's, Tay-Sachs, Sandhoff, Krabbe, Wolman, and Gaucher syndromes. These targeted substrates are based upon fluorescent probes that have a low pKa value for optimum fluorescence at the lower physiological pH values found in the lysosomes as well as targeting groups to direct their accumulation to the lysosomes using a live-cell staining format.
This kit is sold by Marker Gene under product code M1377.
Important Licensing Information: This product may be covered by one or more United States or International Patents and is subject to our Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 units LysoLive™ SulfGreen 10 x 50µl Lysosomal Staining Standard 1 x 100µl
ab253377 has been referenced in 1 publication.
- Harlan FK et al. Fluorogenic Substrates for Visualizing Acidic Organelle Enzyme Activities. PLoS One 11:e0156312 (2016). PubMed: 27228111