Recombinant Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3666] to Lysophospholipase 1/LPL-I
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Lysophospholipase 1/LPL-I antibody [EPR3666]
See all Lysophospholipase 1/LPL-I primary antibodies -
Description
Rabbit monoclonal [EPR3666] to Lysophospholipase 1/LPL-I -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human Lysophospholipase 1/LPL-I aa 50-150.
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Positive control
- WB: HAP1 cell lysate, mouse spleen whole cell lysate; human fetal heart and liver lysate and human spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3666 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 21, 24 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 21, 24 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Has depalmitoylating activity and also low lysophospholipase activity. -
Sequence similarities
Belongs to the AB hydrolase 2 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10434 Human
- Entrez Gene: 18777 Mouse
- Entrez Gene: 25514 Rat
- Omim: 605599 Human
- SwissProt: O75608 Human
- SwissProt: P97823 Mouse
- SwissProt: P70470 Rat
- Unigene: 435850 Human
see all -
Alternative names
- Acyl protein thioesterase 1 antibody
- Acyl-protein thioesterase 1 antibody
- APT 1 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
Lane 3: Mouse spleen whole cell lysate (40 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab91600 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.ab91600 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was observed when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91600 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with ab216773, Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed and ab216776, Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3666] (ab91600) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab91600 has been referenced in 1 publication.
- Vujic I et al. Acyl protein thioesterase 1 and 2 (APT-1, APT-2) inhibitors palmostatin B, ML348 and ML349 have different effects on NRAS mutant melanoma cells. Oncotarget 7:7297-306 (2016). WB . PubMed: 26771141