Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

Overview

  • Product name

    Anti-Lysophospholipase 1/LPL-I antibody [EPR3667]
    See all Lysophospholipase 1/LPL-I primary antibodies
  • Description

    Rabbit monoclonal [EPR3667] to Lysophospholipase 1/LPL-I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Lysophospholipase 1/LPL-I aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: fetal liver, fetal heart, Human spleen, Human liver, Mouse heart, Mouse kidney, Mouse spleen Mouse liver, Rat liver and Rat kidney lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3667
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 25 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
      Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
      Lanes 1 - 2: Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91606 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was obsereved when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE.  ab91606 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution (Purified)

      Lane 1 : Human liver lysates
      Lane 2 : Human fetal heart lysates
      Lane 3 : Mouse liver lysates
      Lane 4 : Rat liver lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa

    • All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : Fetal heart lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 25 kDa

    • All lanes : Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size: 25 kDa

    References

    This product has been referenced in:

    • Kathayat RS  et al. Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes. Nat Commun 9:334 (2018). Read more (PubMed: 29362370) »
    • Sadeghi RS  et al. Wnt5a signaling induced phosphorylation increases APT1 activity and promotes melanoma metastatic behavior. Elife 7:N/A (2018). WB . Read more (PubMed: 29648538) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (muscle)
    Loading amount
    20 µg
    Specification
    muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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