Key features and details
- Mouse monoclonal [1F9] to Lysosomal acid lipase/LAL
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Lysosomal acid lipase/LAL antibody [1F9]
See all Lysosomal acid lipase/LAL primary antibodies
DescriptionMouse monoclonal [1F9] to Lysosomal acid lipase/LAL
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
Recombinant full length protein (GST-tag) corresponding to Human Lysosomal acid lipase/LAL aa 1-399. (AAH12287).
MKMRFLGLVV CLVLWTLHSE GSGGKLTAVD PETNMNVSEI ISYWGFPSEE YLVETEDGYI LCLNRIPHGR KNHSDKGPKP VVFLQHGLLA DSSNWVTNLA NSSLGFILAD AGFDVWMGNS RGNTWSRKHK TLSVSQDEFW AFSYDEMAKY DLPASINFIL NKTGQEQVYY VGHSQGTTIG FIAFSQIPEL AKRIKMFFAL GPVASVAFCT SPMAKLGRLP DHLIKDLFGD KEFLPQSAFL KWLGTHVCTH VILKELCGNL CFLLCGFNER NLNMSRVDVY TTHSPAGTSV QNMLHWSQAV KFQKFQAFDW GSSAKNYFHY NQSYPPTYNV KDMLVPTAVW SGGHDWLADV YDVNILLTQI TNLVFHESIP EWEHLDFIWG LDAPWRLYNK IINLMRKYQ
Database link: P38571
- Human spleen tissue; Lysosomal acid lipase/LAL transfected 293T lysate.
This product was previously labelled as Lysosomal acid lipase
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab219113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
FunctionCrucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation.
Involvement in diseaseDefects in LIPA are the cause of Wolman disease (WOD) [MIM:278000]. WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year.
Defects in LIPA are the cause of cholesteryl ester storage disease (CESD) [MIM:278000]. CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
- Information by UniProt
- Acid cholesteryl ester hydrolase antibody
- CESD antibody
- cholesterol ester hydrolase antibody
All lanes : Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)
Lane 1 : Lysosomal acid lipase/LAL transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Predicted band size: 45 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling Lysosomal acid lipase/LAL with ab219113 at 10µg/ml.
Immunoprecipitation analysis of Lysosomal acid lipase/LAL transfected lysate using ab219113 and Protein A Magnetic Bead, and immunoblotted with Lysosomal acid lipase/LAL rabbit polyclonal antibody
ab219113 has not yet been referenced specifically in any publications.