Key features and details
- Mouse monoclonal [9G7F12] to Lysosomal acid lipase/LAL
- Suitable for: Flow Cyt, WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Lysosomal acid lipase/LAL antibody [9G7F12]
See all Lysosomal acid lipase/LAL primary antibodies
DescriptionMouse monoclonal [9G7F12] to Lysosomal acid lipase/LAL
Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Lysosomal acid lipase/LAL.
This product was previously labelled as Lysosomal acid lipase
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab36597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCrucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation.
Involvement in diseaseDefects in LIPA are the cause of Wolman disease (WOD) [MIM:278000]. WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year.
Defects in LIPA are the cause of cholesteryl ester storage disease (CESD) [MIM:278000]. CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset.
Sequence similaritiesBelongs to the AB hydrolase superfamily. Lipase family.
- Information by UniProt
- Acid cholesteryl ester hydrolase antibody
- CESD antibody
- cholesterol ester hydrolase antibody
All lanes : Anti-Lysosomal acid lipase/LAL antibody [9G7F12] (ab36597)
Lane 1 : Molecular weight marker
Lane 2 : Truncated LAL recombinant protein
Predicted band size: 46 kDa
Observed band size: ~45 kDa why is the actual band size different from the predicted?
ab36597 (2 µg/ml) staining Lysosomal acid lipase/LAL in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is lysosomal staining in the bronchiolar epithelium and resident macrophages .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Overlay histogram showing HepG2 cells stained with ab36597 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36597, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
ab36597 has been referenced in 2 publications.
- Cahova M et al. The increased activity of liver lysosomal lipase in nonalcoholic Fatty liver disease contributes to the development of hepatic insulin resistance. Biochem Res Int 2012:135723 (2012). PubMed: 21904679
- Wang F et al. Role of lysosomal acid lipase in the intracellular metabolism of LDL-transported dehydroepiandrosterone-fatty acyl esters. Am J Physiol Endocrinol Metab 295:E1455-61 (2008). WB ; Human . PubMed: 18796546