Lysosomal Staining Kit - Green Fluorescence (Ex405nm) - Cytopainter (ab112138)

Overview

  • Product name

    Lysosomal Staining Kit - Green Fluorescence (Ex405nm) - Cytopainter
    See all Lysosomes kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


    ab112138 is designed to label lysosomes in live cells with green fluorescence of large Stokes Shift at Ex/Em = 405/520 nm. ab112138 uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator, a hydrophobic compound, easily permeates intact live cells and gets trapped inside the lysosomes. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components. It is suitable for both suspension and adherent cells.


    Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide


     

  • Notes

    ab112138 should be stored desiccated.

  • Platform

    Fluorescence microscope

Properties

Images

  • Image of U2OS cells stained with ab112138, 405 nm Excitation in a black 96-well plate

Protocols

References

ab112138 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting Abcam. The answers to your questions are below: 1. This is basically for all our standard alone fluorescent dyes. For this typical kit, simply store at -20C will be fine (the lyso sensor is DMSO stock solution). 2. We don't have direct experiences on the labeling cells stain with antibody simultaneously. However, the kit is for live cell labeling and the dye can be fixed, so theoretically it should be OK after fixed and then label with Ab. 3. No. it will not. the kit is design for no wash based live cell assay. 4. Yes. 5. As long as the cells are alive. 6. Yes I hope this information helps. Please contact us with any other questions.

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