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1. I see a storage note that it is recommended to store dessicated. Does that simply mean to store with dessicant? Do you recommend any particular dessicant? 2. Does the dye interfere with antibody labeling, for instance for sorting or analysis of live cells by flow cytometry? 3. Will components of tissue culture media such as phenol red interfere with the signal? 4. We are assuming this will only label lysosomes of live cells. Is that correct? 5. How long will the dye be detectable after labeling cells? 6. Will the dye remain associated with lysosomes after fixation of cells?
Asked on Oct 31 2011
Thank you for contacting Abcam. The answers to your questions are below: 1. This is basically for all our standard alone fluorescent dyes. For this typical kit, simply store at -20C will be fine (the lyso sensor is DMSO stock solution). 2. We don't have direct experiences on the labeling cells stain with antibody simultaneously. However, the kit is for live cell labeling and the dye can be fixed, so theoretically it should be OK after fixed and then label with Ab. 3. No. it will not. the kit is design for no wash based live cell assay. 4. Yes. 5. As long as the cells are alive. 6. Yes I hope this information helps. Please contact us with any other questions.
Answered on Oct 31 2011