Lysosomal Staining Kit - Orange - Cytopainter (ab138895)

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Fluorescence Microscopy of HeLa cells

    Key features and details

    • Assay type: Cell-based
    • Detection method: Fluorescent
    • Platform: Fluorescence microscope
    • Sample type: Adherent cells, Suspension cells

    Overview

    • Product name

      Lysosomal Staining Kit - Orange - Cytopainter
      See all Lysosomes kits

    • Detection method

      Fluorescent

    • Sample type

      Adherent cells, Suspension cells

    • Assay type

      Cell-based

    • Product overview

      Abcam fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.


      ab138895 is designed to label lysosomes of live cells in orange fluorescence at Ex/Em = 540/560 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes.


      The LysoOrange Indicator dye used in the kit has extremely high photostability as well as excellent cellular retention makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.


      Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide

    • Notes

      Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.

    • Platform

      Fluorescence microscope

    Properties

    Images

    • Fluorescence Microscopy of HeLa cells
      Fluorescence Microscopy of HeLa cells
      HeLa cells stained with ab138895 in a black 96-well plate. The TRTIC signals were compared at 0 seconds (upper image) and 120 seconds (lower image) exposure time by using a fluorescence microscope.

    References (2)

    Publishing research using ab138895? Please let us know so that we can cite the reference in this datasheet.

    ab138895 has been referenced in 2 publications.

    • Sano M  et al. Cellular Responses of Human Lymphatic Endothelial Cells to Carbon Nanomaterials. Nanomaterials (Basel) 10:N/A (2020). PubMed: 32674394
    • Nomura H  et al. Specific biological responses of the synovial membrane to carbon nanotubes. Sci Rep 5:14314 (2015). FM . PubMed: 26388047

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