Overview

  • Product name
    Lysosomal Staining Reagent - Deep Red - Cytopainter
    See all Lysosome kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (qualitative)
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Abcam's Lysosomal Staining Reagent - Deep Red | Cytopainter (ab176829) is part of a series of new fluorogenic probes to label lysosomes of live cells. The proprietary lysotropic dye selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry.


    CytoPainter LysoDeep Red Indicator Reagent (ab176829) has extremely high photostability as well as excellent cellular retention making it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.

  • Notes

    Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.

  • Tested applications
    Suitable for: FMmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.

Images

  • Image of HeLa cells stained with Abcam's CytoPainter LysoDeep Red Indicator Reagent (ab176829) in a Costar black 96-well plate. The TRTIC signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.

Protocols

References

ab176829 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
We performed lysosome staining on Hydractinia echinata, a marine invertebrate.
The animals were incubates for 1.5 hours in 1:1000 dilution. Animals were then fixed in 4% PFA in PBS and stained with Hoescht at 1:2000 dilution in PBS before being mounted in 90% glycerol.
The picture shows staining in the tentacles(hoescht in blue, mitochondria in red)

Mr. James Gahan

Verified customer

Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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