The animals were incubates for 1.5 hours in 1:1000 dilution. Animals were then fixed in 4% PFA in PBS and stained with Hoescht at 1:2000 dilution in PBS before being mounted in 90% glycerol.
The picture shows staining in the tentacles(hoescht in blue, mitochondria in red)
Mr. James Gahan
Submitted Feb 28 2014