Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Product nameLysosomal Staining Reagent - Green - Cytopainter
See all Lysosomes kits
Sample typeAdherent cells, Suspension cells
Assay typeCell-based (qualitative)
Species reactivityReacts with: Mammals, Other species
Abcam's Lysosomal Staining Reagent - Green | Cytopainter (ab176826) is part of a series of new fluorogenic probes to label lysosomes of live cells. The proprietary lysotropic dye selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and becomes trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5 – 4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains the pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests CytoPainter LysoGreen Indicator Reagent 1 x 500 tests
- lysosome marker
ab176826 has not yet been referenced specifically in any publications.