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  1. Link

    lysosomal-staining-reagent-orange-cytopainter-ab176827.pdf

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Tags & Cell Markers Subcellular Markers Organelles Lysosome
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Lysosomal Staining Reagent - Orange - Cytopainter (ab176827)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1) Submit a question References (2)

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Image of HeLa cells stained with CytoPainter LysoOrange Indicator Reagent (ab176827)
  • Emission and Excitation spectra for CytoPainter LysoOrange Indicator Reagent (ab176827).

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Overview

  • Product name

    Lysosomal Staining Reagent - Orange - Cytopainter
    See all Lysosomes kits
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (qualitative)
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Abcam's Lysosomal Staining Reagent - Orange | Cytopainter (ab176827) is part of a series of new fluorogenic probes to label lysosomes of live cells. The proprietary lysotropic dye selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and becomes trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.


    Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide

  • Notes

    Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 500 tests
    CytoPainter LysoOrange Indicator Reagent 1 x 500 tests
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Lysosome Staining Kits
  • Alternative names

    • lysosome marker
    • lysotracker

Associated products

  • Corresponding kit

    • Lysosomal Staining Kit - Orange - Cytopainter (ab138895)

Images

  • Image of HeLa cells stained with CytoPainter LysoOrange Indicator Reagent (ab176827)
    Image of HeLa cells stained with CytoPainter LysoOrange Indicator Reagent (ab176827)

    Image of HeLa cells stained with Abcam's CytoPainter LysoOrange Indicator Reagent (ab176827) in a Costar black 96-well plate using a fluorescence microscope TRITC Channel.

  • Emission and Excitation spectra for CytoPainter LysoOrange Indicator Reagent (ab176827).
    Emission and Excitation spectra for CytoPainter LysoOrange Indicator Reagent (ab176827).

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab176827? Please let us know so that we can cite the reference in this datasheet.

ab176827 has been referenced in 2 publications.

  • Lu Y  et al. Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator. Cells 8:N/A (2019). PubMed: 31430912
  • Wang G  et al. Label-Free Fluorescent Poly(amidoamine) Dendrimer for Traceable and Controlled Drug Delivery. Biomacromolecules 20:2148-2158 (2019). PubMed: 30995832

Customer reviews and Q&As

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Lysosomes marker

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
U87 cell culture, human glioblastome, just add cytopainter orange 1/1000 30 minutes. Wash twice et use for imaging.

Mrs. Francoise Geffroy

Verified customer

Submitted Sep 22 2014

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