Lysosomal Staining Reagent - Orange - Cytopainter (ab176827)
Overview
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Product name
Lysosomal Staining Reagent - Orange - Cytopainter
See all Lysosomes kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Lysosomal Staining Reagent - Orange | Cytopainter (ab176827) is part of a series of new fluorogenic probes to label lysosomes of live cells. The proprietary lysotropic dye selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and becomes trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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Notes
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests CytoPainter LysoOrange Indicator Reagent 1 x 500 tests -
Research areas
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Alternative names
- lysosome marker
- lysotracker
Associated products
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Corresponding kit
Images
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Image of HeLa cells stained with CytoPainter LysoOrange Indicator Reagent (ab176827)
Image of HeLa cells stained with Abcam's CytoPainter LysoOrange Indicator Reagent (ab176827) in a Costar black 96-well plate using a fluorescence microscope TRITC Channel.
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Emission and Excitation spectra for CytoPainter LysoOrange Indicator Reagent (ab176827).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab176827 has been referenced in 2 publications.
- Lu Y et al. Internalization Characterization of Si Nanorod with Camouflaged Cell Membrane Proteins Reveals ATXN2 as a Negative Regulator. Cells 8:N/A (2019). PubMed: 31430912
- Wang G et al. Label-Free Fluorescent Poly(amidoamine) Dendrimer for Traceable and Controlled Drug Delivery. Biomacromolecules 20:2148-2158 (2019). PubMed: 30995832