Lysosome Isolation Kit (ab234047)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Lysosome Isolation Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K235 Lysosome Purification Kit. K235-50 is the same size as the 50 test size of ab234047.
Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 50 tests Lysosome Enrichment Buffer 1 x 100ml Density Gradient Media II 1 x 85ml Lysosome Isolation Buffer 1 x 25ml Protease Inhibitor Cocktail II 1 x 1ml
Images
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab234047 has been referenced in 12 publications.
- Nadeem A et al. Protein-lipid interaction at low pH induces oligomerization of the MakA cytotoxin from Vibrio cholerae. Elife 11:N/A (2022). PubMed: 35131030
- Jung S et al. Inhibitory role of TRIP-Br1 oncoprotein in anticancer drug-mediated programmed cell death via mitophagy activation. Int J Biol Sci 18:3859-3873 (2022). PubMed: 35813469
- Dong L et al. Mannose ameliorates experimental colitis by protecting intestinal barrier integrity. Nat Commun 13:4804 (2022). PubMed: 35974017
- Chu TT et al. Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C. Nature 596:570-575 (2021). PubMed: 34290407
- Guo S et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12:42 (2021). PubMed: 33414446