Overview

  • Product name
    Lysosome Isolation Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Product overview

    Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.

  • Notes

    Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 50 tests
    Lysosome Enrichment Buffer 1 x 100ml
    Lysosome Gradient 1 x 85ml
    Lysosome Isolation Buffer 1 x 25ml
    Protease Inhibitor Cocktail 1 x 1ml

Images

  • Tentative Lysosome band location in gradient density.

  • Data is normalized by the amount of protein used per well.

Protocols

References

ab234047 has not yet been referenced specifically in any publications.

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