Overview

  • Product name

    Lysosome/ER/Nuclear Staining Reagent - Cytopainter
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Assay time

    0h 30m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Lysosome/ER/Nuclear Staining Reagent | Cytopainter (ab139488) containsOrganelle Reagent II, a mixture of cell-permeable red fluorescent lysosomal dye, green fluorescent endoplasmic reticulum dye and blue fluorescent nucleic acid dye. The staining pattern arising from the combination of these three dyes permits visualization of the target organelles by fluorescence/confocal microscopy. The reagent, supplied as a 500X solution, is sufficient for 1000 microscopy assays. The single-tube format makes this multi-organelle stain reagent easy to use.


    Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide

  • Notes

    Lysosomal Staining - Red: Ex/Em = 568/667 nm

    Endoplasmic Reticulum Staining - Green: Ex/Em = 440/565 nm

    Nuclear Staining - Blue: Ex/Em = 350/461 nm

  • Platform

    Fluorescence microscope

Properties

Images

  • Live cells treated with Chloroquine and stained with Organelle Reagent II showing composite of (A) lysosomes in red, (B) endoplasmic reticula in green, and (C) nuclei in blue.

Protocols

References

ab139488 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



I am sorry the cytopainter lysosome dyes have not yet been tested on fixed tissue or live cells that have ben fixed post staining. Therefore, I am sorry but we really cannot comment on how successful this may be for we do not know how well the dye may continue to stain once the cells are fixed.

I can recommend to consider using a marker antibody instead. For example we have the following well characterized antibodies that I hope may be helpful:

Anti-LAMP1 antibody - Lysosome Marker (ab24170)
Description:Rabbit polyclonal to LAMP1 - Lysosome Marker
Application: ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB
Reactivity: Mouse, Rat, Chicken, Hamster, Cat, Dog, Human, Xenopus laevis, Zebrafish (predicted: Cow)
https://www.abcam.com/index.html?datasheet=24170 (or use the following: https://www.abcam.com/index.html?datasheet=24170).


Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (ab124767)
Description: Rabbit monoclonal [EPR6599] to M6PR (cation independent) - Lysosome
Application: Flow Cyt, ICC/IF, IHC-P, IP, WB
Reactivity: Mouse, Rat, Human
https://www.abcam.com/index.html?datasheet=124767 (or use the following: https://www.abcam.com/index.html?datasheet=124767).

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up