Overview

  • Product name

    Lysozyme Activity Assay Kit (Fluorometric)
    See all Lysozyme kits
  • Detection method

    Fluorescent
  • Sample type

    Saliva, Serum, Cell culture extracts, Cell Lysate, Tissue Homogenate, Purified protein, Tissue Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    0.002 U/ml
  • Product overview

    Lysozyme Activity Assay Kit (ab211113) provides a convenient method for quantifying Lysozyme activity in mammalian cell or tissue lysates, biological fluids such as tears or saliva. This product can also be used to measure lysozyme activity in yeast and bacteria. The assay is based on the ability of lysozyme to cleave a synthetic substrate and release a free fluorophore which can be easily quantified at Ex/Em= 360/445 nm in a fluorescent microplate reader.


    This kit provides a simple, ultra-sensitive assay that can detect as low as 2 µU/mL of Lysozyme activity.

  • Notes

    Lysozyme (EC 3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the β-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. It exhibits antibacterial, antitumor and immune modulatory activities. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis and nephritis.

  • Platform

    Microplate reader

Properties

Images

  • Typical 4-Methylumbelliferon (4-MU) Standard Curve.

  • Measurement of Lysozyme activity in human fluids. Assays were performed following kit protocols.

  • Measurement of Lysozyme activity in human cultured cell lysates. Assays were performed following kit protocols.

Protocols

References

ab211113 has not yet been referenced specifically in any publications.

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