Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Reviews (3)Q&A (4)References (53)
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
  • Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2994(2)] to Lysozyme
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Lysozyme antibody [EPR2994(2)]
    See all Lysozyme primary antibodies

  • Description

    Rabbit monoclonal [EPR2994(2)] to Lysozyme

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1, HepG2, RAW 264.7 and HL-60 whole cell lysate; Human spleen tissue lysate; Natural human Lysozyme protein. IHC-P: Human tonsil, spleen, lung, kidney, brain, breast and heart tissues; Mouse spleen and small intestine tissues. ICC/IF: THP-1 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/250.

 For unpurified use at 1/500 - 1/1000.
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P (3)
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

See IHC antigen retrieval protocols.
Notes
ICC/IF
1/250.

 For unpurified use at 1/500 - 1/1000.
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98°C, below boiling, and then let cool for 10-20 min.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

    • Involvement in disease

      Amyloidosis 8

    • Sequence similarities

      Belongs to the glycosyl hydrolase 22 family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • 1 4 beta N acetylmuramidase C antibody
        • 1 antibody
        • 4-beta-N-acetylmuramidase C antibody
        • EC 3.2.1.17 antibody
        • LYSC_HUMAN antibody
        • Lysosyme antibody
        • Lysozyme (renal amyloidosis) antibody
        • Lysozyme C antibody
        • Lysozyme C precursor antibody
        • LYZ antibody
        • LZM antibody
        • Renal amyloidosis antibody
        see all

      Images

      • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        All lanes : Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution

        Lane 1 : Wild-type THP1 cell lysate
        Lane 2 : LYZ knockout THP1 cell lysate
        Lane 3 : HepG2 cell lysate
        Lane 4 : HeLa cell lysate

        Lysates/proteins at 20 µg per lane.

        Performed under reducing conditions.

        Predicted band size: 17 kDa
        Observed band size: 16 kDa why is the actual band size different from the predicted?



        Lanes 1 - 4: Merged signal (red and green). Green - ab108508 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

        ab108508 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab108508 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Immunohistochemical analysis of paraffin-embedded human lung tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody.Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

        Secondary
        Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

        Predicted band size: 17 kDa
        Observed band size: 16 kDa why is the actual band size different from the predicted?



        Blocking/Diluting buffer 5% NFDM/TBST
      • Immunocytochemistry/ Immunofluorescence - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunocytochemistry/ Immunofluorescence - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia) cells labeling lysozyme with purified ab108508 at 1/250. Cells were fixed with 100% methanol. ab150077, Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 g/mL) - Microtubule Marker (Alexa Fluor®594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)This image is courtesy of an Abreview submitted by Mr. Thorsten Cramer.

        Paraffin-embedded mouse small intestine tissue stained for Lysozyme using ab108508 in immunohistochemical analysis.

        See Abreview

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

      • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        All lanes : Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution (unpurified)

        Lane 1 : HL60 (human promyelocytic leukemia cell line) cell lysate
        Lane 2 : Human spleen lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 17 kDa

      • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/50000 dilution (purified) + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 20 µg

        Secondary
        Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

        Predicted band size: 17 kDa
        Observed band size: 16 kDa why is the actual band size different from the predicted?



        Blocking/Diluting buffer 5% NFDM/TBST
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1 µg/ml (unpurified) + Native human Lysozyme protein (ab91125) at 0.1 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 17 kDa


        Exposure time: 4 minutes
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Unpurified ab108508 showing negative staining in Normal human brain tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Unpurified ab108508 showing negative staining in Normal human breast tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

        Unpurified ab108508 showing negative staining in Normal human heart tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Lysozyme antibody [EPR2994(2)] (ab108508)
        Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

      References (53)

      Publishing research using ab108508? Please let us know so that we can cite the reference in this datasheet.

      ab108508 has been referenced in 53 publications.

      • Xie Y  et al. Impact of a high-fat diet on intestinal stem cells and epithelial barrier function in middle-aged female mice. Mol Med Rep 21:1133-1144 (2020). PubMed: 32016468
      • Bergkvist L  et al. Serum amyloid P component promotes formation of distinct aggregated lysozyme morphologies and reduces toxicity in Drosophila flies expressing F57I lysozyme. PLoS One 15:e0227227 (2020). PubMed: 31978114
      • He D  et al. Gut stem cell aging is driven by mTORC1 via a p38 MAPK-p53 pathway. Nat Commun 11:37 (2020). PubMed: 31896747
      • Wang Y  et al. Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. J Exp Med 217:N/A (2020). PubMed: 31753849
      • Sim H  et al. Iroquois Homeobox Protein 2 Identified as a Potential Biomarker for Parkinson's Disease. Int J Mol Sci 21:N/A (2020). PubMed: 32422864
      View all Publications for this product

      Customer reviews and Q&As

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      1-3 of 3 Abreviews

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Small intestine)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate (pH 6.0)
      Permeabilization
      No
      Specification
      Small intestine
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Mr. Brett Bell

      Verified customer

      Submitted Dec 28 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
      Permeabilization
      No
      Specification
      Spleen
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 31 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (small intestine)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate
      Permeabilization
      No
      Specification
      small intestine
      Blocking step
      AB block Dako X0590 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      10% NBF
      Read More

      Mr. Thorsten Cramer

      Verified customer

      Submitted Aug 18 2016

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