Name: Anti-Lysozyme antibody [EPR2994(2)]
Brand: RabMAb
SKU: ab108508
Rating: 5 (4 reviews)

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Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2994(2)] to Lysozyme
Suitable for: ICC/IF, WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Biotin Carrier Free HRP

Product name

Anti-Lysozyme antibody [EPR2994(2)]
See all Lysozyme primary antibodies
Description

Rabbit monoclonal [EPR2994(2)] to Lysozyme
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: THP-1, HepG2, RAW 264.7 and HL-60 whole cell lysate; Human spleen tissue lysate; Natural human Lysozyme protein. IHC-P: Human tonsil, spleen, lung, kidney, brain, breast and heart tissues; Mouse spleen and small intestine tissues. ICC/IF: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2994(2)
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human LYZ (Lysozyme) knockout THP-1 cell line (ab269595)
KO cell lysates
- Human LYZ (Lysozyme) knockout THP-1 cell lysate (ab270711)
Positive Controls
- HL-60 whole cell lysate (ab7914)
Recombinant Protein
- Recombinant Human Lysozyme protein (ab158839)
Related Products
- Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/250. For unpurified use at 1/500 - 1/1000.
WB		1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P	(4)	1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. See IHC antigen retrieval protocols.

Notes
ICC/IF 1/250. For unpurified use at 1/500 - 1/1000.
WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. See IHC antigen retrieval protocols.

Application notes

Is unsuitable for IP.

Function

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Involvement in disease

Amyloidosis 8
Sequence similarities

Belongs to the glycosyl hydrolase 22 family.
Cellular localization

Secreted.
Target information above from: UniProt accession P61626 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4069 Human
- Entrez Gene: 17105 Mouse
- Entrez Gene: 17110 Mouse
- Omim: 153450 Human
- SwissProt: P61626 Human
- SwissProt: P08905 Mouse
- Unigene: 524579 Human
Alternative names
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
- EC 3.2.1.17 antibody
- LYSC_HUMAN antibody
- Lysosyme antibody
- Lysozyme (renal amyloidosis) antibody
- Lysozyme C antibody
- Lysozyme C precursor antibody
- LYZ antibody
- LZM antibody
- Renal amyloidosis antibody
see all

Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

All lanes : Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution

Lane 1 : Wild-type THP1 cell lysate
Lane 2 : LYZ knockout THP1 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab108508 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab108508 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab108508 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Immunohistochemical analysis of paraffin-embedded human lung tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody.Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia) cells labeling lysozyme with purified ab108508 at 1/250. Cells were fixed with 100% methanol. ab150077, Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 g/mL) - Microtubule Marker (Alexa Fluor^®594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)This image is courtesy of an Abreview submitted by Mr. Thorsten Cramer.

Paraffin-embedded mouse small intestine tissue stained for Lysozyme using ab108508 in immunohistochemical analysis.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue sections labeling lysozyme with purified ab108508 at a dilution of 1/1500 (0.6 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

All lanes : Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/10000 dilution (unpurified)

Lane 1 : HL60 (human promyelocytic leukemia cell line) cell lysate
Lane 2 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 17 kDa
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1/50000 dilution (purified) + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 17 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Anti-Lysozyme antibody [EPR2994(2)] (ab108508) at 1 µg/ml (unpurified) + Native human Lysozyme protein (ab91125) at 0.1 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 17 kDa

Exposure time: 4 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Unpurified ab108508, at 1/1000 dilution, staining Lysozyme in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Unpurified ab108508 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Unpurified ab108508 showing negative staining in Normal human breast tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Unpurified ab108508 showing negative staining in Normal human heart tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Lysozyme antibody [EPR2994(2)] (ab108508)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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Publishing research using ab108508? Please let us know so that we can cite the reference in this datasheet.

ab108508 has been referenced in 62 publications.

Yagishita Y et al. Constitutive Activation of Nrf2 in Mice Expands Enterogenesis in Small Intestine Through Negative Regulation of Math1. Cell Mol Gastroenterol Hepatol 11:503-524 (2021). PubMed: 32896624
Liu Y et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev 2021:6683836 (2021). PubMed: 33688393
Arora K et al. Patient personalized translational tools in cystic fibrosis to transform data from bench to bed-side and back. Am J Physiol Gastrointest Liver Physiol 320:G1123-G1130 (2021). PubMed: 33949881
Farrall AL et al. PWD/Ph-Encoded Genetic Variants Modulate the Cellular Wnt/ß-Catenin Response to Suppress ApcMin-Triggered Intestinal Tumor Formation. Cancer Res 81:38-49 (2021). PubMed: 33154092
Krüger J et al. Drug Inhibition of SARS-CoV-2 Replication in Human Pluripotent Stem Cell-Derived Intestinal Organoids. Cell Mol Gastroenterol Hepatol 11:935-948 (2021). PubMed: 33186749

View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (COLON)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Specification

COLON

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 25 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Small intestine)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate (pH 6.0)

Permeabilization

Specification

Small intestine

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Brett Bell

Verified customer

Submitted Dec 28 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Spleen)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0

Permeabilization

Specification

Spleen

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 31 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (small intestine)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate

Permeabilization

Specification

small intestine

Blocking step

AB block Dako X0590 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Thorsten Cramer

Verified customer

Submitted Aug 18 2016

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Positive Controls

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (62)

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lysozyme antibody [EPR2994(2)]