Overview

  • Product name

    Anti-Lysozyme antibody [EPR2995]
    See all Lysozyme primary antibodies
  • Description

    Rabbit monoclonal [EPR2995] to Lysozyme
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lysozyme aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HL-60 cell lysate and HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab91653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
    • Involvement in disease

      Amyloidosis 8
    • Sequence similarities

      Belongs to the glycosyl hydrolase 22 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1 4 beta N acetylmuramidase C antibody
      • 1 antibody
      • 4-beta-N-acetylmuramidase C antibody
      • EC 3.2.1.17 antibody
      • LYSC_HUMAN antibody
      • Lysosyme antibody
      • Lysozyme (renal amyloidosis) antibody
      • Lysozyme C antibody
      • Lysozyme C precursor antibody
      • LYZ antibody
      • LZM antibody
      • Renal amyloidosis antibody
      see all

    Images

    • Anti-Lysozyme antibody [EPR2995] (ab91653) at 1/1000 dilution + HL-60 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    • ab91653 at 1/1000 dilution staining Lysozyme in HeLa cells

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for providing that information.

    I have had a look at the two rabbit monoclonal against Lysozyme (ab91653 and ab108508). Both of these antibodies should be able to detect all three forms of the protein (the wild type as well as your two mutants). The immunogen used to raise the ab108508 antibody has been taken from a region N-terminal of your mutations, whilst the immunogen used to raise the antibody ab91653 is from the C-terminal domain. Neither immunogen overlaps any of the mutation sites.

    I hope this information has beenof help. If you have any further questions please do not hesitate to ask.

    Read More

    Answer

    Thank you for contacting us. Based on sequence analysis, immunogen of ab91653 does not share any significant sequence similarity with chicken. For the immunogen of ab108508, it shares 67% identity with chicken Lysozyme C, so it should also be human specific. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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