Recombinant Anti-Lysozyme antibody [SP329] (ab223503)
![Western blot - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-451752-anti-lysozyme-antibody-sp329-western-blot-thp1-wt-thp1-lyz-knockout-hepg2-hela.jpg "Western blot - Anti-Lysozyme antibody [SP329] (ab223503)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP329] to Lysozyme
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Lysozyme antibody [SP329]
See all Lysozyme primary antibodies -
Description
Rabbit monoclonal [SP329] to Lysozyme -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit
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Immunogen
Recombinant full length protein corresponding to Human Lysozyme.
Database link: P61626 -
Positive control
- IHC-P: Human tonsil, thymus, liver, spleen, lung, pancreatic carcinoma, and stomach carcinoma tissue; WB: THP-1, HepG2 and HL-60 cell lysate; Flow Cyt: HL-60 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP329 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/400. Predicted molecular weight: 16 kDa.
Primary antibody incubation for 1 hour at room temperature. |
|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
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| Flow Cyt |
1/400.
Primary antibody incubation for 30 minutes at 4°C. |
| Notes |
|---|
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WB
1/400. Predicted molecular weight: 16 kDa. Primary antibody incubation for 1 hour at room temperature. |
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature. |
|
Flow Cyt
1/400. Primary antibody incubation for 30 minutes at 4°C. |
Target
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Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
Involvement in disease
Amyloidosis 8 -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- SwissProt: P16973 Rabbit
- Unigene: 524579 Human
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Alternative names
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
Images
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Western blot - Anti-Lysozyme antibody [SP329] (ab223503)All lanes : Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution
Lane 1 : Wild-type THP1 cell lysate
Lane 2 : LYZ knockout THP1 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16 kDaLanes 1 - 4: Merged signal (red and green). Green - ab223503 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab223503 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab223503 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-11-AntiLysozyme-antibody-SP329-IHC-Human-stomach-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-10-AntiLysozyme-antibody-SP329-IHC-Human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290756-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
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![Western blot - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290754-anti-lysozyme-antibody-sp329-western-blot.jpg)
Western blot - Anti-Lysozyme antibody [SP329] (ab223503)Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate
Predicted band size: 16 kDa -
![Flow Cytometry - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290752-anti-lysozyme-antibody-sp329-flow-cytometry.jpg)
Flow Cytometry - Anti-Lysozyme antibody [SP329] (ab223503)Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab223503 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290751-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human thymus tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290755-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290749-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290757-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290758-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)](https://www.abcam.com/ps/products/223/ab223503/Images/ab223503-290734-anti-lysozyme-antibody-sp329-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223503 has not yet been referenced specifically in any publications.