Overview

  • Product name

    Anti-Lysozyme antibody [SP329]
    See all Lysozyme primary antibodies
  • Description

    Rabbit monoclonal [SP329] to Lysozyme
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit
  • Immunogen

    Recombinant full length protein corresponding to Human Lysozyme.
    Database link: P61626

  • Positive control

    • IHC-P: Human tonsil, thymus, liver, spleen, lung, pancreatic carcinoma, and stomach carcinoma tissue; WB: HL-60 cell lysate; FC: HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 16 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Flow Cyt 1/400.

Primary antibody incubation for 30 minutes at 4°C.

Target

  • Function

    Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in disease

    Amyloidosis 8
  • Sequence similarities

    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

  • Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate

    Predicted band size: 16 kDa

  • Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab223503 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

References

ab223503 has not yet been referenced specifically in any publications.

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