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  1. Link

    lysozyme-antibody-sp329-ab223503.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Lysozyme antibody [SP329] (ab223503)

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Western blot - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Western blot - Anti-Lysozyme antibody [SP329] (ab223503)
  • Flow Cytometry - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP329] to Lysozyme
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-Lysozyme antibody [SP329]
    See all Lysozyme primary antibodies
  • Description

    Rabbit monoclonal [SP329] to Lysozyme
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit
  • Immunogen

    Recombinant full length protein corresponding to Human Lysozyme.
    Database link: P61626

  • Positive control

    • IHC-P: Human tonsil, thymus, liver, spleen, lung, pancreatic carcinoma, and stomach carcinoma tissue; WB: THP-1, HepG2 and HL-60 cell lysate; Flow Cyt: HL-60 cells.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    SP329
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Cardiovascular
    • Blood
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Lysosome

Associated products

  • Alternative Versions

    • Anti-Lysozyme antibody [SP329] - BSA and Azide free (ab242420)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HL-60 whole cell lysate (ab7914)
  • Recombinant Protein

    • Recombinant Human Lysozyme protein (ab158839)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/400. Predicted molecular weight: 16 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Flow Cyt
1/400.

Primary antibody incubation for 30 minutes at 4°C.

Notes
WB
1/400. Predicted molecular weight: 16 kDa.

Primary antibody incubation for 1 hour at room temperature.

IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Flow Cyt
1/400.

Primary antibody incubation for 30 minutes at 4°C.

Target

  • Function

    Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in disease

    Amyloidosis 8
  • Sequence similarities

    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P61626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4069 Human
    • Omim: 153450 Human
    • SwissProt: P61626 Human
    • SwissProt: P16973 Rabbit
    • Unigene: 524579 Human
    • Alternative names

      • 1 4 beta N acetylmuramidase C antibody
      • 1 antibody
      • 4-beta-N-acetylmuramidase C antibody
      • EC 3.2.1.17 antibody
      • LYSC_HUMAN antibody
      • Lysosyme antibody
      • Lysozyme (renal amyloidosis) antibody
      • Lysozyme C antibody
      • Lysozyme C precursor antibody
      • LYZ antibody
      • LZM antibody
      • Renal amyloidosis antibody
      see all

    Images

    • Western blot - Anti-Lysozyme antibody [SP329] (ab223503)
      Western blot - Anti-Lysozyme antibody [SP329] (ab223503)
      All lanes : Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution

      Lane 1 : Wild-type THP1 cell lysate
      Lane 2 : LYZ knockout THP1 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 16 kDa
      Observed band size: 16 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab223503 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

      ab223503 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab223503 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    • Western blot - Anti-Lysozyme antibody [SP329] (ab223503)
      Western blot - Anti-Lysozyme antibody [SP329] (ab223503)
      Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate

      Predicted band size: 16 kDa

    • Flow Cytometry - Anti-Lysozyme antibody [SP329] (ab223503)
      Flow Cytometry - Anti-Lysozyme antibody [SP329] (ab223503)

      Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab223503 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human thymus tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (ab223503)

      Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab223503? Please let us know so that we can cite the reference in this datasheet.

    ab223503 has not yet been referenced specifically in any publications.

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