Overview

  • Product name

    Anti-Lysozyme antibody [SP329] - BSA and Azide free
    See all Lysozyme primary antibodies
  • Description

    Rabbit monoclonal [SP329] to Lysozyme - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit
  • Immunogen

    Recombinant full length protein corresponding to Human Lysozyme.
    Database link: P61626

  • Positive control

    • IHC-P: Human tonsil, thymus, liver, spleen, lung, pancreatic carcinoma, and stomach carcinoma tissue.
  • General notes

    Ab242420 is the carrier-free version of ab223503. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242420 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab242420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Primary antibody incubation for 30 minutes at 4°C.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.

Primary antibody incubation for 1 hour at room temperature.

Target

  • Function

    Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in disease

    Amyloidosis 8
  • Sequence similarities

    Belongs to the glycosyl hydrolase 22 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223503)
  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab223503 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223503).

References

ab242420 has not yet been referenced specifically in any publications.

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