Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP350] to Lysozyme - C-terminal
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
Product nameAnti-Lysozyme antibody [SP350] - C-terminal
See all Lysozyme primary antibodies
DescriptionRabbit monoclonal [SP350] to Lysozyme - C-terminal
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Lysozyme (C terminal).
Database link: P61626
- WB: HL-60 whole cell lysate. IHC-P: Human tonsil, lung, liver, spleen, stomach adenocarcinoma and pancreatic adenocarcinoma tissues. Flow Cyt: HL-60 cells.
FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab227708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/400. Predicted molecular weight: 16 kDa.|
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.
FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Involvement in diseaseAmyloidosis 8
Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
- Information by UniProt
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate
Predicted band size: 16 kDa
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab227708 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab227708 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227708 has not yet been referenced specifically in any publications.