Anti-LysRS antibody (ab155114)
Key features and details
- Rabbit polyclonal to LysRS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LysRS antibody
See all LysRS primary antibodies -
Description
Rabbit polyclonal to LysRS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human LysRS aa 280-597.
Database link: Q15046 -
Positive control
- H1299 whole cell lysate; HeLa cells; Human NCI-N87 xenograft tissue.
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General notes
Store as concentrated solution.Previously labelled as Lysyl tRNA synthetase.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 68 kDa. | |
| IHC-P | 1/100 - 1/1000. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)] | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. -
Involvement in disease
Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. -
Domain
The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2. -
Cellular localization
Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha. - Information by UniProt
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Database links
- Entrez Gene: 3735 Human
- Entrez Gene: 85305 Mouse
- Entrez Gene: 292028 Rat
- Omim: 601421 Human
- SwissProt: Q15046 Human
- SwissProt: Q99MN1 Mouse
- Unigene: 3100 Human
- Unigene: 196544 Mouse
see all -
Alternative names
- CMTRIB antibody
- DFNB89 antibody
- EC 6.1.1.6 antibody
see all
Images
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Western blot - Anti-LysRS antibody (ab155114)Anti-LysRS antibody (ab155114) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody (ab155114)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling LysRs with ab155114 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody (ab155114)Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue, labeling LysRs with ab155114 at 1/100 dilution.
Protocols
References (0)
ab155114 has not yet been referenced specifically in any publications.
