Overview

  • Product name
  • Description
    Rabbit polyclonal to LysRS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human LysRS aa 280-597.
    Database link: Q15046

  • Positive control
    • H1299 whole cell lysate; HeLa cells; Human NCI-N87 xenograft tissue.
  • General notes
    Store as concentrated solution.

    Previously labelled as Lysyl tRNA synthetase. 

Properties

Applications

Our Abpromise guarantee covers the use of ab155114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000. suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • Involvement in disease
    Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Domain
    The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
  • Cellular localization
    Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
  • Information by UniProt
  • Database links
  • Alternative names
    • CMTRIB antibody
    • DFNB89 antibody
    • EC 6.1.1.6 antibody
    • KARS 1 antibody
    • KARS 2 antibody
    • KARS antibody
    • KARS1 antibody
    • KARS2 antibody
    • KIAA0070 antibody
    • KRS antibody
    • Lysine tRNA ligase antibody
    • Lysine--tRNA ligase antibody
    • LysRS antibody
    • Lysyl tRNA synthetase antibody
    • Lysyl-tRNA synthetase antibody
    • SYK_HUMAN antibody
    see all

Images

  • Anti-LysRS antibody (ab155114) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling LysRs with ab155114 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue, labeling LysRs with ab155114 at 1/100 dilution.

References

ab155114 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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