Overview

  • Product name

    Anti-LysRS antibody [EPR7920]
    See all LysRS primary antibodies
  • Description

    Rabbit monoclonal [EPR7920] to LysRS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human LysRS aa 1-100 (N terminal).
    Database link: Q15046

  • Positive control

    • Jurkat, HeLa, 293T, HepG2 and Caco 2 cell lysates; Human breast carcinoma tissue; HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Lysyl tRNA synthetase. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • Involvement in disease

    Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Domain

    The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
  • Cellular localization

    Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMTRIB antibody
    • DFNB89 antibody
    • EC 6.1.1.6 antibody
    • KARS 1 antibody
    • KARS 2 antibody
    • KARS antibody
    • KARS1 antibody
    • KARS2 antibody
    • KIAA0070 antibody
    • KRS antibody
    • Lysine tRNA ligase antibody
    • Lysine--tRNA ligase antibody
    • LysRS antibody
    • Lysyl tRNA synthetase antibody
    • Lysyl-tRNA synthetase antibody
    • SYK_HUMAN antibody
    see all

Images

  • All lanes : Anti-LysRS antibody [EPR7920] (ab134912) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of LysRs in paraffin embedded Human breast carcinoma tissue, using ab134912 at a 1/50 dilution.

  • Immunofluorescent analysis of LysRs in HeLa cells, using ab134912 at a 1/250 dilution.

References

This product has been referenced in:

  • Hei Z  et al. Retractile lysyl-tRNA synthetase-AIMP2 assembly in the human multi-aminoacyl-tRNA synthetase complex. J Biol Chem 294:4775-4783 (2019). Read more (PubMed: 30733335) »
See 1 Publication for this product

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