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    lysrs-antibody-n-terminal-ab228486.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs
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Anti-LysRS antibody - N-terminal (ab228486)

  • Datasheet
  • SDS
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Western blot - Anti-LysRS antibody - N-terminal (ab228486)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody - N-terminal (ab228486)

Key features and details

  • Rabbit polyclonal to LysRS - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-LysRS antibody - N-terminal
    See all LysRS primary antibodies
  • Description

    Rabbit polyclonal to LysRS - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human LysRS (N terminal). The exact sequence is proprietary.
    Database link: Q15046

  • Positive control

    • WB: A549 whole cell lysate. IHC-P: Human breast cancer tissue.
  • General notes

    Previously labelled as Lysyl tRNA synthetase. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)

Applications

Our Abpromise guarantee covers the use of ab228486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • Involvement in disease

    Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Domain

    The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
  • Cellular localization

    Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
  • Target information above from: UniProt accession Q15046 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3735 Human
    • Entrez Gene: 714516 Rhesus monkey
    • Omim: 601421 Human
    • SwissProt: Q15046 Human
    • Unigene: 3100 Human
    • Alternative names

      • CMTRIB antibody
      • DFNB89 antibody
      • EC 6.1.1.6 antibody
      • KARS 1 antibody
      • KARS 2 antibody
      • KARS antibody
      • KARS1 antibody
      • KARS2 antibody
      • KIAA0070 antibody
      • KRS antibody
      • Lysine tRNA ligase antibody
      • Lysine--tRNA ligase antibody
      • LysRS antibody
      • Lysyl tRNA synthetase antibody
      • Lysyl-tRNA synthetase antibody
      • SYK_HUMAN antibody
      see all

    Images

    • Western blot - Anti-LysRS antibody - N-terminal (ab228486)
      Western blot - Anti-LysRS antibody - N-terminal (ab228486)
      Anti-LysRS antibody - N-terminal (ab228486) at 1/1000 dilution + A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

      Developed using the ECL technique.

      Predicted band size: 68 kDa



      7.5% SDS-PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody - N-terminal (ab228486)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody - N-terminal (ab228486)

      Formalin-fixed, paraffin-embedded human breast cancer tissue stained for LysRs with ab228486 at 1/500 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab228486? Please let us know so that we can cite the reference in this datasheet.

    ab228486 has not yet been referenced specifically in any publications.

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