Key features and details
- Rabbit polyclonal to LYVE1
- Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Dog, Human, Recombinant fragment
- Isotype: IgG
Product nameAnti-LYVE1 antibody
See all LYVE1 primary antibodies
DescriptionRabbit polyclonal to LYVE1
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Dog, Human, Recombinant fragment
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Please reconstitue in sterile water to a concentration of 0.1-10mg/ml. When reconstituted the antibody is stable for at least two weeks at 2-8°C.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-P||Use a concentration of 6 - 30 µg/ml.|
|IHC-Fr||Use a concentration of 6 - 30 µg/ml.|
|Flow Cyt||Use a concentration of 3 - 10 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionLigand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
Tissue specificityMainly expressed in endothelial cells lining lymphatic vessels.
Sequence similaritiesContains 1 Link domain.
Cellular localizationMembrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
- Information by UniProt
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.
The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.
Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.
ab36993 has been referenced in 11 publications.
- Gage BK et al. Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Cell Stem Cell 27:254-269.e9 (2020). IHC-P, Flow Cyt ; Human . PubMed: 32640183
- Ha SK et al. Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels. Bio Protoc 8:N/A (2018). PubMed: 29780855
- Olmeda D et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature 546:676-680 (2017). PubMed: 28658220
- Zhu C et al. Sulfatase 2 facilitates lymphangiogenesis in breast cancer by regulating VEGF-D. Oncol Rep 36:3161-3171 (2016). WB ; Human . PubMed: 27748846
- Louveau A et al. Structural and functional features of central nervous system lymphatic vessels. Nature 523:337-41 (2015). IHC . PubMed: 26030524