Anti-LYVE1 antibody (ab36993)

ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not specifically directed towards the C-terminal of LYVE-1.
However, we have Anti-LYVE1 antibody (ab33682) which is guaranteed to work in IHC-P and it's immunogen is a synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Mouse LYVE1.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 123456.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your swift reply.

I am sorry to hear that the cause of the whole trouble is the attachment; technique is supposed to make ones' live easier not harder.

I am happy to send you your replacement antibody out on Monday, but I would need to know some crucial protocol information from you for our records.

So far, the information I recieved from the previous emails were about the species you have used, and the antigen retrieval buffers. However, could you please shortly clarify the following key factors:

What human tissue is tested?

Duration of retrieval?

Blocking conditions (x% BSA/Serum in TSB/PBS for 1h/ overnight)?

Primary antibody incubation conditions?


As stated before, this is usually a very reliable antibody, and I would like to emphasize that our replacement is as well covered by our guarantee. However, if you prefer to get an alternative product, please let me know what kind of applications and species besides IHC-P, ICC and human it should work with and I will search our catalog for a matching product.


Thank you very much, and please let me know if you would prefer a different product to be send to you.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for your email.

I have checked our records and saw that we are still waiting for you to return our questionnaire to us. With this questionnaire we can get a good overview of the customer's experiment and parameters, and provide individual help on a case-to-case basis. We often find that suggesting some scientifically thought out optimization tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimize the results first.

If we cannot provide any tips or if the tips do not work, we'll gladly replace the product or provide a credit note or refund.

The questionnaire for the Abpromise guarantee is also an important tool for us as it represents a base for in-house investigations to target and resolve the problems that customers can experience as quickly as possible.

I would like to assure you that this a very reliable antibody usually, as we had only another complaint since 2007 with over 450 sold units.

As you have informed us two month after the purchase of this product, you are still covered by our Abpromise.

However, it is still important that we get the protocol information for our files as well.

Please find the questionnaire attached to this email



I thank you for your understanding and cooperation. I am looking forward receiving your reply.

Thank you for your reply.

Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days). However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.

The questionnaire for the Abpromise guarantee is also an important tool for us as it represents a base for in-house investigations to target and resolve the problems that customers can experience as quickly as possible.

Therefore, if you could fill out the questionnaire so we can getan overview of your experiments, that wouldbe highly appreciated and will speed up the process.

I am looking forward to receive your reply.


1) Abcam product code ab

2) Abcam order reference number or product batch number

3) Description of the problem

4) Sample preparation:
Species
Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other:
Sample preparation
Positive control
Negative control

5) Fixation step
Yes/No
If yes: Fixative agent and concentration
Fixation time
Fixation temperature

6) Antigen retrieval method

7) Permeabilization method:
Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
Permeabilizing agent and concentration:


8) Blocking agent (eg BSA, serum…):
Concentration
Blocking time
Blocking temperature

9) Endogenous peroxidases blocked?
Endogenous biotins blocked?

10) Primary antibody (If more than one was used, describe in “additional notes”) :
Concentration or dilution
Diluent buffer
Incubation time

11) Secondary antibody:
Species:
Reacts against:
Concentration or dilution
Diluent buffer
Incubation time
Fluorochrome or enzyme conjugate

12) Washing after primary and secondary antibodies:
Buffer
Number of washes

13) Detection method

14) How many times have you run this staining?
Do you obtain the same results every time?
What steps have you altered to try and optimize the use of this antibody?

Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.