Overview

  • Product name
  • Description
    Rabbit polyclonal to LYVE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Recombinant fragment corresponding to Human LYVE1 aa 24-232. Recombinant human soluble LYVE1 fragment from insect cells (ab54341).
    (Peptide available as ab54341)

Properties

  • Form
    Lyophilised:Please reconstitue in sterile water to a concentration of 0.1-10mg/ml. When reconstituted the antibody is stable for at least two weeks at 2-8°C.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Recombinant Human LYVE1 protein (ab54341).
IHC-P Use a concentration of 6 - 30 µg/ml.
IHC-Fr Use a concentration of 6 - 30 µg/ml.
Flow Cyt Use a concentration of 3 - 10 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificity
    Mainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similarities
    Contains 1 Link domain.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Images

  • IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.
  • The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.

  • All lanes : Anti-LYVE1 antibody (ab36993)

    Lane 1 : Recombinant Human LYVE1 protein (ab54341)
    Lane 2 : Recombinant Mouse LYVE1 protein (ab54342)

    Predicted band size: 35 kDa

  • Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.

References

This product has been referenced in:
  • Ha SK  et al. Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels. Bio Protoc 8:N/A (2018). Read more (PubMed: 29780855) »
  • Olmeda D  et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature 546:676-680 (2017). Read more (PubMed: 28658220) »
See all 10 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human umbilical vein endothelial cells (HUVEC))
Permeabilization
No
Specification
Human umbilical vein endothelial cells (HUVEC)
Blocking step
BSA + 5% Fetal bovine serum + 5% human serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 40 min 100C
Specification
breast cancer
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 13 2015

Answer


ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not specifically directed towards the C-terminal of LYVE-1.
However, we have Anti-LYVE1 antibody (ab33682) which is guaranteed to work in IHC-P and it's immunogen is a synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Mouse LYVE1.

Read More

Question

Thank you for your replay.

That will be really helpful if you will send us the replacement (ab10278), and am really hoping it will give us beautiful staining results.

Always I would like to get the good antibody for IF staining. Since mouse LYVE1 antibody from your company works very well, so I ordered ab36993.

But it didn’t work for paraffin slide in IF staining for any condition.

(It may work for frozen, but I couldn’t get the human patient frozen sample.)

So I would like to try another human LYVE1 antibody from your company.





The key factors you need to know is here.

What human tissue is tested?---human H&N patient paraffin slides

Duration of retrieval?----10 minutes in pressure cooker

Blocking conditions (x% BSA/Serum in TSB/PBS for 1h/ overnight)?----10% Serum in 0.01% tween TBS

Primary antibody incubation conditions?-----4 degree over night



Hope this information is enough. Please let me know if you need to know more about my staining condition.



Thank you for your help!



Masako







From: technical@abcam.com [mailto:technical@abcam.com]
Sent: Friday, November 30, 2012 9:07 AM
To: Oka, Masako
Subject: Reply from Abcam to your enquiry regarding ab36993 [CCE4412194]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Dr Oka

We have an answer to your inquiry:

Thank you for your swift reply.

I am sorry to hear that the cause of the whole trouble is the attachment; technique is supposed to make ones' live easier not harder.

I am happy to send you your replacement antibody out on Monday, but I would need to know some crucial protocol information from you for our records.

So far, the information I recieved from the previous emails were about the species you have used, and the antigen retrieval buffers. However, could you please shortly clarify the following key factors:

What human tissue is tested?

Duration of retrieval?

Blocking conditions (x% BSA/Serum in TSB/PBS for 1h/ overnight)?

Primary antibody incubation conditions?


As stated before, this is usually a very reliable antibody, and I would like to emphasize that our replacement is as well covered by our guarantee. However, if you prefer to get an alternative product, please let me know what kind of applications and species besides IHC-P, ICC and human it should work with and I will search our catalog for a matching product.


Thank you very much, and please let me know if you would prefer a different product to be send to you.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447



Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=4412194


Best regards,
Cathrin

Cathrin Zeeck
Scientific Support Specialist
Abcam plc
https://www.abcam.com

* Sie können mich auch gerne auf Deutsch kontaktieren


Your original inquiry to Abcam:














I answered the questions with previous e-mail, I believe.

Somehow it was hard to put my information on the word file your company attached before,

so I gave all answer with using the e-mail your company gave me before.

Could you please check it? But if you can’t accept the style, I will try to do it again, but, hope you can get enough information from my previous e-mail.



Thank you for your help.








Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE4412194]













Discover more at abcam.com

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 123456.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your swift reply.

I am sorry to hear that the cause of the whole trouble is the attachment; technique is supposed to make ones' live easier not harder.

I am happy to send you your replacement antibody out on Monday, but I would need to know some crucial protocol information from you for our records.

So far, the information I recieved from the previous emails were about the species you have used, and the antigen retrieval buffers. However, could you please shortly clarify the following key factors:

What human tissue is tested?

Duration of retrieval?

Blocking conditions (x% BSA/Serum in TSB/PBS for 1h/ overnight)?

Primary antibody incubation conditions?


As stated before, this is usually a very reliable antibody, and I would like to emphasize that our replacement is as well covered by our guarantee. However, if you prefer to get an alternative product, please let me know what kind of applications and species besides IHC-P, ICC and human it should work with and I will search our catalog for a matching product.


Thank you very much, and please let me know if you would prefer a different product to be send to you.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for your email.

I have checked our records and saw that we are still waiting for you to return our questionnaire to us. With this questionnaire we can get a good overview of the customer's experiment and parameters, and provide individual help on a case-to-case basis. We often find that suggesting some scientifically thought out optimization tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimize the results first.

If we cannot provide any tips or if the tips do not work, we'll gladly replace the product or provide a credit note or refund.

The questionnaire for the Abpromise guarantee is also an important tool for us as it represents a base for in-house investigations to target and resolve the problems that customers can experience as quickly as possible.

I would like to assure you that this a very reliable antibody usually, as we had only another complaint since 2007 with over 450 sold units.

As you have informed us two month after the purchase of this product, you are still covered by our Abpromise.

However, it is still important that we get the protocol information for our files as well.

Please find the questionnaire attached to this email



I thank you for your understanding and cooperation. I am looking forward receiving your reply.

Read More

Answer

Thank you for your reply.

Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days). However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.

The questionnaire for the Abpromise guarantee is also an important tool for us as it represents a base for in-house investigations to target and resolve the problems that customers can experience as quickly as possible.

Therefore, if you could fill out the questionnaire so we can getan overview of your experiments, that wouldbe highly appreciated and will speed up the process.

I am looking forward to receive your reply.


1) Abcam product code ab

2) Abcam order reference number or product batch number

3) Description of the problem

4) Sample preparation:
Species
Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other:
Sample preparation
Positive control
Negative control

5) Fixation step
Yes/No
If yes: Fixative agent and concentration
Fixation time
Fixation temperature

6) Antigen retrieval method

7) Permeabilization method:
Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
Permeabilizing agent and concentration:


8) Blocking agent (eg BSA, serum…):
Concentration
Blocking time
Blocking temperature

9) Endogenous peroxidases blocked?
Endogenous biotins blocked?

10) Primary antibody (If more than one was used, describe in “additional notes”) :
Concentration or dilution
Diluent buffer
Incubation time

11) Secondary antibody:
Species:
Reacts against:
Concentration or dilution
Diluent buffer
Incubation time
Fluorochrome or enzyme conjugate

12) Washing after primary and secondary antibodies:
Buffer
Number of washes

13) Detection method

14) How many times have you run this staining?
Do you obtain the same results every time?
What steps have you altered to try and optimize the use of this antibody?

Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem.

Read More

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Specification
Skin
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0,05%
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C

Mr. Álvaro Teijeira

Verified customer

Submitted Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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