Overview

  • Product name
    Anti-LYVE1 antibody
    See all LYVE1 primary antibodies
  • Description
    Rabbit polyclonal to LYVE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Recombinant fragment corresponding to Human LYVE1 aa 24-232. Recombinant human soluble LYVE1 fragment from insect cells (ab54341).
    (Peptide available as ab54341)

Properties

  • Form
    Lyophilised:Please reconstitue in sterile water to a concentration of 0.1-10mg/ml. When reconstituted the antibody is stable for at least two weeks at 2-8°C.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Recombinant Human LYVE1 protein (ab54341).
IHC-P Use a concentration of 6 - 30 µg/ml.
IHC-Fr Use a concentration of 6 - 30 µg/ml.
Flow Cyt Use a concentration of 3 - 10 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificity
    Mainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similarities
    Contains 1 Link domain.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localization
    Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Database links
    • Alternative names
      • Cell surface retention sequence-binding protein 1 antibody
      • CRSBP 1 antibody
      • CRSBP-1 antibody
      • CRSBP1 antibody
      • extracellular link domain containing 1 antibody
      • extracellular link domain-containing 1 antibody
      • Extracellular link domain-containing protein 1 antibody
      • HAR antibody
      • Hyaluronic acid receptor antibody
      • Lymphatic endothelium specific hyaluronan receptor antibody
      • lymphatic vessel endothelial hyaluronan receptor 1 antibody
      • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
      • LYVE 1 antibody
      • LYVE-1 antibody
      • LYVE1 antibody
      • LYVE1_HUMAN antibody
      • XLKD1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)
      IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.
    • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab36993)
      Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab36993)
      The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.

    • Western blot - Anti-LYVE1 antibody (ab36993)
      Western blot - Anti-LYVE1 antibody (ab36993)
      All lanes : Anti-LYVE1 antibody (ab36993)

      Lane 1 : Recombinant Human LYVE1 protein (ab54341)
      Lane 2 : Recombinant Mouse LYVE1 protein (ab54342)

      Predicted band size: 35 kDa

    • Flow Cytometry - Anti-LYVE1 antibody (ab36993)
      Flow Cytometry - Anti-LYVE1 antibody (ab36993)

      Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.

    References

    This product has been referenced in:
    • Ha SK  et al. Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels. Bio Protoc 8:N/A (2018). Read more (PubMed: 29780855) »
    • Zhu C  et al. Sulfatase 2 facilitates lymphangiogenesis in breast cancer by regulating VEGF-D. Oncol Rep 36:3161-3171 (2016). WB ; Human . Read more (PubMed: 27748846) »

    See all 9 Publications for this product

    Publishing research using ab36993? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    9 reviews or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LYVE1 antibody

     Excellent
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human umbilical vein endothelial cells (HUVEC))
    Permeabilization
    No
    Specification
    Human umbilical vein endothelial cells (HUVEC)
    Blocking step
    BSA + 5% Fetal bovine serum + 5% human serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Mar 24 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-LYVE1 antibody

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 40 min 100C
    Specification
    breast cancer
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jul 13 2015

    Hi! Is this antibody C-terminal? If not than do you have C-ternimal (cytoplasmic domain) antibody against human LYVE-1 for IHC-P?


    ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not specifically directed towards the C-...

    Read More

    Thank you for your replay.

    That will be really helpful if you will send us the replacement (ab10278), and am really hoping it will give us beautiful staining results.

    Always I would like to get the good antibody for IF staining. Since...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

    Read More

    I answered the questions with previous e-mail, I believe.

    Somehow it was hard to put my information on the word file your company attached before,

    so I gave all answer with using the e-mail your company gave me before.

    Coul...

    Read More

    Thank you for your swift reply.

    I am sorry to hear that the cause of the whole trouble is the attachment; technique is supposed to make ones' live easier not harder.

    I am happy to send you your replacement antibody out on Monday, but ...

    Read More

    Thank you for your e-mail and sorry for my late response.

    I tried to make sure if any condition can work with this antibody (LYVE1 ab36993), but still hard to get clear staining results.

    Sorry, it was taking time for making sure, so m...

    Read More

    Thank you for your email.

    I have checked our records and saw that we are still waiting for you to return our questionnaire to us. With this questionnaire we can get a good overview of the customer's experiment and parameters, and provide indiv...

    Read More

    Thank you so much for your replay.

    We bought the antibody 2 months ago, so I hope we can do something.

    So….is it possible to ask you to replace to ab10278?

    Thank you for your reply.

    Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the ...

    Read More

    I have a question about the primary antibody from your company.




    I bought the primary antibody for human LYVE1 ab36993, but I couldn’t get staining with human sample.

    I did IF and IHC both, and also tried to...

    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    I a...

    Read More

    Immunohistochemistry (Frozen sections) abreview for Anti-LYVE1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 0,05%
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    abreview image
    Username

    Mr. Álvaro Teijeira

    Verified customer

    Submitted Aug 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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