• Product name
  • Description
    Rabbit polyclonal to LYVE1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Dog, Human
  • Immunogen

    Recombinant fragment corresponding to Human LYVE1 aa 24-232. Recombinant human soluble LYVE1 fragment from insect cells (ab54341).
    (Peptide available as ab54341)


  • Form
    Lyophilised:Please reconstitue in sterile water to a concentration of 0.1-10mg/ml. When reconstituted the antibody is stable for at least two weeks at 2-8°C.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab36993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Recombinant Human LYVE1 protein (ab54341).
IHC-P Use a concentration of 6 - 30 µg/ml.
IHC-Fr Use a concentration of 6 - 30 µg/ml.
Flow Cyt Use a concentration of 3 - 10 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Function
    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificity
    Mainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similarities
    Contains 1 Link domain.
  • Post-translational
  • Cellular localization
    Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all


  • IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.
  • The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.

  • All lanes : Anti-LYVE1 antibody (ab36993)

    Lane 1 : Recombinant Human LYVE1 protein (ab54341)
    Lane 2 : Recombinant Mouse LYVE1 protein (ab54342)

    Predicted band size: 35 kDa

  • Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.


This product has been referenced in:
  • Ha SK  et al. Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels. Bio Protoc 8:N/A (2018). Read more (PubMed: 29780855) »
  • Zhu C  et al. Sulfatase 2 facilitates lymphangiogenesis in breast cancer by regulating VEGF-D. Oncol Rep 36:3161-3171 (2016). WB ; Human . Read more (PubMed: 27748846) »

See all 9 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Human umbilical vein endothelial cells (HUVEC))
Human umbilical vein endothelial cells (HUVEC)
Blocking step
BSA + 5% Fetal bovine serum + 5% human serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 24 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 40 min 100C
breast cancer
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 13 2015

ab36993's immunogen is a recombinant full length protein produced in insect cells, corresponding to amino acids 24-232 of Human LYVE1 and is fused to a C-terminal His-tag (6xHis). This antibody is therefore not specifically directed towards the C-...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

Thank you for your swift reply.

I am sorry to hear that the cause of the whole trouble is the attachment; technique is supposed to make ones' live easier not harder.

I am happy to send you your replacement antibody out on Monday, but ...

Read More

Thank you for your email.

I have checked our records and saw that we are still waiting for you to return our questionnaire to us. With this questionnaire we can get a good overview of the customer's experiment and parameters, and provide indiv...

Read More

Thank you for your reply.

Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the ...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (Skin)
Yes - Triton 0,05%
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C

Mr. Álvaro Teijeira

Verified customer

Submitted Aug 03 2012


Sign up