Anti-LYVE1 antibody (ab36993)
Key features and details
- Rabbit polyclonal to LYVE1
- Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Dog, Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LYVE1 antibody
See all LYVE1 primary antibodies -
Description
Rabbit polyclonal to LYVE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Dog, Human, Recombinant fragment -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Please reconstitue in sterile water to a concentration of 0.1-10mg/ml. When reconstituted the antibody is stable for at least two weeks at 2-8°C. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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| IHC-P | (1) |
Use a concentration of 6 - 30 µg/ml.
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| IHC-Fr | (1) |
Use a concentration of 6 - 30 µg/ml.
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| Flow Cyt |
Use a concentration of 3 - 10 µg/ml.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
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IHC-P
Use a concentration of 6 - 30 µg/ml. |
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IHC-Fr
Use a concentration of 6 - 30 µg/ml. |
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Flow Cyt
Use a concentration of 3 - 10 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
Tissue specificity
Mainly expressed in endothelial cells lining lymphatic vessels. -
Sequence similarities
Contains 1 Link domain. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 10894 Human
- Omim: 605702 Human
- SwissProt: Q9Y5Y7 Human
- Unigene: 655332 Human
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Alternative names
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)IHC with paraffin-embedded sections of human intestine (border area of a colon carcinoma) using antibody ab36993.
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Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab36993)The staining was performed on frozen sections from human colon carcinoma using ab36993 (polyclonal anti-human LYVE-1 antibody; protein-A purified; 10 µg/ml). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of malignant canine mammary tumor tissue sections labelling LYVE1 with ab36993.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of benign canine mammary tumor tissue sections labelling LYVE1 with ab36993.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab36993)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of lymphatic vessels of normal canine mammary tissue sections labelling LYVE1 with ab36993.
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Western blot - Anti-LYVE1 antibody (ab36993) -
Flow Cytometry - Anti-LYVE1 antibody (ab36993)Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab36993.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab36993 has been referenced in 11 publications.
- Gage BK et al. Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Cell Stem Cell 27:254-269.e9 (2020). IHC-P, Flow Cyt ; Human . PubMed: 32640183
- Ha SK et al. Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels. Bio Protoc 8:N/A (2018). PubMed: 29780855
- Olmeda D et al. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine. Nature 546:676-680 (2017). PubMed: 28658220
- Zhu C et al. Sulfatase 2 facilitates lymphangiogenesis in breast cancer by regulating VEGF-D. Oncol Rep 36:3161-3171 (2016). WB ; Human . PubMed: 27748846
- Louveau A et al. Structural and functional features of central nervous system lymphatic vessels. Nature 523:337-41 (2015). IHC . PubMed: 26030524