Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 40 min 100C
Specification
breast cancer
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: DAKO diluent
Dilution
1/25
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine anti-mouse anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Magnetic beads
Host species: Goat
Clonality: Polyclonal
Conjugation: Magnetic beads
Additional data
Additional Notes
Antibody does not lead itself to automation, and even with overnight incubation staining of lymphatic endothelium is very weak staining
Abcam user community
Verified customer
Submitted Jul 13 2015