Anti-LYVE1 antibody - BSA and Azide free (ab14917)
Key features and details
- Rabbit polyclonal to LYVE1 - BSA and Azide free
- Suitable for: ICC, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-LYVE1 antibody - BSA and Azide free
See all LYVE1 primary antibodies -
Description
Rabbit polyclonal to LYVE1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment (His-tag) corresponding to Mouse LYVE1 aa 1-250 (C terminal).
Database link: Q8BHC0 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein-A Chromatography (+his tag depleted). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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IHC-P | (27) |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
Tissue specificity
Mainly expressed in endothelial cells lining lymphatic vessels. -
Sequence similarities
Contains 1 Link domain. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 114332 Mouse
- SwissProt: Q8BHC0 Mouse
- Unigene: 396078 Mouse
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Alternative names
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse intestine tissue staining LYVE-1 with ab14917. Positive staining is shown in the lymphatic endothelial cells (red).
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Immunocytochemistry/immunofluorecent analysis of mouse colon tissue labelling LYVE-1 with ab14917 (red).
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At the time point of 75dpi spirochetes (Bb) were not observed in association with the lymphatic-like vessels (LYVE-1) that run parallel to the sagittal sinus (SS, arrow) of the dura mater.
Dura samples were collected from transcardially perfused mice by craniotomy and post-fixed in 4% paraformaldehyde for 24h at 4°C. Samples were permeabilized in 0.1% Triton X-100, washed 3 times, and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block. For B. burgdorferi staining, each sample was incubated in 1:100 dilution of rat anti-mouse unconjugated monoclonal anti-CD31 IgG, and 1:50 dilution biotinylated rabbit anti-B. burgdorferi polyclonal IgG at 4°C overnight. On the following day, the samples were washed, and stained with 1:100 dilution of Alexa 555 goat anti-rat polyclonal IgG, and 1:200 dilution of Alexa 488 streptavidin
for 1 hour at room temperature, covered from light. Secondary antibody-only controls for B. burgdorferi indirect fluorescent assay were performed in vitro and no fluorescence was observed.Some of the dura samples were also stained for lymphatic vessels in a separate step, using 1:200 ab14917, followed by washing and secondary staining with 1:200 Alexa 633 goat-anti rabbit polyclonal IgG (yellow).
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ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.
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ab14917 staining LYVE1 in mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-buffer pH 9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (256)
ab14917 has been referenced in 256 publications.
- McNamara NB et al. Microglia regulate central nervous system myelin growth and integrity. Nature 613:120-129 (2023). PubMed: 36517604
- Zhao J et al. Delivery of costimulatory blockade to lymph nodes promotes transplant acceptance in mice. J Clin Invest 132:N/A (2022). PubMed: 36519543
- Ang PS et al. The growth and expansion of meningeal lymphatic networks are affected in craniosynostosis. Development 149:N/A (2022). PubMed: 34908123
- Chen D et al. Angiogenesis depends upon EPHB4-mediated export of collagen IV from vascular endothelial cells. JCI Insight 7:N/A (2022). PubMed: 35015735
- Ishida K et al. Glymphatic system clears extracellular tau and protects from tau aggregation and neurodegeneration. J Exp Med 219:N/A (2022). PubMed: 35212707