Recombinant Anti-LYVE1 antibody [EPR21857] (ab219556)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)](https://www.abcam.com/ps/products/219/ab219556/Images/ab219556-304298-anti-lyve1-antibody-epr21857-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21857] to LYVE1
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LYVE1 antibody [EPR21857]
See all LYVE1 primary antibodies -
Description
Rabbit monoclonal [EPR21857] to LYVE1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human lymph node, brain, fetal lung and fetal spleen lysates. IHC-P: Human colon, tonsil and liver tissue. IP: Human fetal lung lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21857 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 50-70 kDa (predicted molecular weight: 35 kDa).
|
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| IHC-P | (3) |
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/20.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 50-70 kDa (predicted molecular weight: 35 kDa). |
|
IHC-P
1/20000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/20. |
Target
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Function
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
Tissue specificity
Mainly expressed in endothelial cells lining lymphatic vessels. -
Sequence similarities
Contains 1 Link domain. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 10894 Human
- Omim: 605702 Human
- SwissProt: Q9Y5Y7 Human
- Unigene: 655332 Human
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Alternative names
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human tonsil (PMID: 11377291) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)](https://www.abcam.com/ps/products/219/ab219556/Images/ab219556-304297-anti-lyve1-antibody-epr21857-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphatic endothelial cells of human colon (PMID: 11377291) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-LYVE1 antibody [EPR21857] (ab219556)](https://www.abcam.com/ps/products/219/ab219556/Images/ab219556-304294-anti-lyve1-antibody-epr21857-western-blot.jpg)
Western blot - Anti-LYVE1 antibody [EPR21857] (ab219556)All lanes : Anti-LYVE1 antibody [EPR21857] (ab219556) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human lymph node lysate
Lane 3 : Human fetal spleen lysate
Lane 4 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 50-70 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST
Exposure times:
Lane 1: 37 seconds
Lanes 2 & 4: 92 seconds
Lane 3: 6 seconds
Negative control: Human brain (PMID: 20599941).
Bands observed are consistent with full-length and soluble fragments, as described in the literature (PMID: 29262593, PMID: 26966180, PMID: 26926389).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)](https://www.abcam.com/ps/products/219/ab219556/Images/ab219556-304299-anti-lyve1-antibody-epr21857-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21857] (ab219556)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling LYVE1 with ab219556 at 1/20,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on blood sinusoids of human liver (PMID: 11719431) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (ab219556)](https://www.abcam.com/ps/products/219/ab219556/Images/ab219556-304295-anti-lyve1-antibody-epr21857-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LYVE1 antibody [EPR21857] (ab219556)LYVE1 was immunoprecipitated from 0.35 mg of human fetal lung lysate with ab219556 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab219556 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Human fetal lung lysate 10 μg (Input).
Lane 2: ab219556 IP in human fetal lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219556 in human fetal lung lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds.The band observed is consistent with the soluble LYVE1 described in the literature (PMID: 29262593; PMID: 26966180; PMID: 26926389).
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![Anti-LYVE1 antibody [EPR21857] (ab219556)](https://www.abcam.com/ps/products/219/ab219556/Images/ab219556-6-benefits-of-recombinant-antibodies.png)
Anti-LYVE1 antibody [EPR21857] (ab219556)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab219556 has not yet been referenced specifically in any publications.
