Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval (S1699)
Permeabilization
No
Specification
Breast cancer
Blocking step
Pro-block (PBK500) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
6 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Histofine Simple Stain PO(MULTI)
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
We would like to thank the reviewer for submitting this AbReview for this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Oct 05 2018