Overview

  • Product name

  • Description

    Rabbit polyclonal to LZIC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Recombinant fragment within Human LZIC (internal sequence). The exact sequence is proprietary.
    Database link: Q8WZA0

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysates. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-LZIC antibody (ab229149) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    12% SDS-PAGE gel.

  • All lanes : Anti-LZIC antibody (ab229149) at 1/5000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
    Lane 3 : IMR32 (Human brain neuroblast cell line) whole cell lysate
    Lane 4 : SK-N-AS (Human brain neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    12% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LZIC (green) using ab229149 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229149 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229149.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up