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Developmental Biology Organogenesis Gut development Liver development
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RecombinantRabMAb

Recombinant Anti-LZP antibody [EPR9349] (ab172973)

  • Datasheet
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Western blot - Anti-LZP antibody [EPR9349] (ab172973)
  • Flow Cytometry - Anti-LZP antibody [EPR9349] (ab172973)
  • Anti-LZP antibody [EPR9349] (ab172973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9349] to LZP
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-LZP antibody [EPR9349]
    See all LZP primary antibodies
  • Description

    Rabbit monoclonal [EPR9349] to LZP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    within Human LZP aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WWZ8

  • Positive control

    • Human plasma, Human fetal liver and HepG2 lysates. Permeabilized HepG2 cells.
  • General notes

     This product was previously labelled as OIT3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9349
  • Isotype

    IgG
  • Research areas

    • Developmental Biology
    • Organogenesis
    • Gut development
    • Liver development

Associated products

  • Alternative Versions

    • Anti-LZP antibody [EPR9349] - BSA and Azide free (ab249742)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
Notes
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance

      LZP contains one EGF-like domain and one ZP domain. It may be involved in hepatocellular function and development. LZP is liver-specific and is expressed only in the hepatocytes; down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. There are two named isoforms.
    • Cellular localization

      Nucleus envelope. Can be secreted into blood.
    • Database links

      • Entrez Gene: 170392 Human
      • Omim: 609330 Human
      • SwissProt: Q8WWZ8 Human
      • Alternative names

        • Liver specific zona pellucida domain containing protein antibody
        • Liver specific ZP domain containing protein antibody
        • LZP antibody
        • Oncoprotein induced transcript 3 antibody

      Images

      • Western blot - Anti-LZP antibody [EPR9349] (ab172973)
        Western blot - Anti-LZP antibody [EPR9349] (ab172973)
        All lanes : Anti-LZP antibody [EPR9349] (ab172973) at 1/1000 dilution

        Lane 1 : Human Plasma
        Lane 2 : Human fetal liver lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 60 kDa

      • Flow Cytometry - Anti-LZP antibody [EPR9349] (ab172973)
        Flow Cytometry - Anti-LZP antibody [EPR9349] (ab172973)

        Flow cytometric analysis of permeabilized HepG2 cells labeling LZP with ab172973 at 1/100 diution  (red) or a rabbit IgG (negative) (green).

      • Anti-LZP antibody [EPR9349] (ab172973)
        Anti-LZP antibody [EPR9349] (ab172973)

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab172973? Please let us know so that we can cite the reference in this datasheet.

      ab172973 has not yet been referenced specifically in any publications.

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