RecombinantRabMAb

Recombinant Anti-LZP antibody [EPR9349] (ab172973)

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Western blot - Anti-LZP antibody [EPR9349] (ab172973)
  • Flow Cytometry - Anti-LZP antibody [EPR9349] (ab172973)
  • Anti-LZP antibody [EPR9349] (ab172973)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9349] to LZP
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-LZP antibody [EPR9349]
    See all LZP primary antibodies

  • Description

    Rabbit monoclonal [EPR9349] to LZP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    within Human LZP aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WWZ8

  • Positive control

    • Human plasma, Human fetal liver and HepG2 lysates. Permeabilized HepG2 cells.

  • General notes

     This product was previously labelled as OIT3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9349

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
Notes
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Relevance

      LZP contains one EGF-like domain and one ZP domain. It may be involved in hepatocellular function and development. LZP is liver-specific and is expressed only in the hepatocytes; down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. There are two named isoforms.

    • Cellular localization

      Nucleus envelope. Can be secreted into blood.

    • Database links

      • Alternative names

        • Liver specific zona pellucida domain containing protein antibody
        • Liver specific ZP domain containing protein antibody
        • LZP antibody
        • Oncoprotein induced transcript 3 antibody

      Images

      • Western blot - Anti-LZP antibody [EPR9349] (ab172973)
        Western blot - Anti-LZP antibody [EPR9349] (ab172973)
        All lanes : Anti-LZP antibody [EPR9349] (ab172973) at 1/1000 dilution

        Lane 1 : Human Plasma
        Lane 2 : Human fetal liver lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 60 kDa

      • Flow Cytometry - Anti-LZP antibody [EPR9349] (ab172973)
        Flow Cytometry - Anti-LZP antibody [EPR9349] (ab172973)

        Flow cytometric analysis of permeabilized HepG2 cells labeling LZP with ab172973 at 1/100 diution  (red) or a rabbit IgG (negative) (green).

      • Anti-LZP antibody [EPR9349] (ab172973)
        Anti-LZP antibody [EPR9349] (ab172973)

      References (0)

      Publishing research using ab172973? Please let us know so that we can cite the reference in this datasheet.

      ab172973 has not yet been referenced specifically in any publications.

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