Key features and details
- Rabbit polyclonal to LZTFL1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-LZTFL1 antibody
See all LZTFL1 primary antibodies
DescriptionRabbit polyclonal to LZTFL1
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Orangutan
Recombinant fragment corresponding to Human LZTFL1 aa 116-299.
EFEKAEITSSNKKPILDVTKPKLAPLNEGGTAELLNKEILRLQEENEKLK SRLKTIEIQATNALDEKSKLEKALQDLQLDQGNQKDFIKAQDLSNLENTV AALKSEFQKTLNDKTENQKSLEENLATAKHDLLRVQEQLHMAEKELEKKF QQTAAYRNMKEILTKKNDQIKDLRKRLAQYEPED
Database link: Q9NQ48
- WB: HEK-293T, HepG2, HeLa and U-251 MG whole cell lysates. IHC-P: Human testis tissue. ICC/IF: U-251 MG cells. IP: HepG2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 35 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
- Information by UniProt
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All lanes : Anti-LZTFL1 antibody (ab236795) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Paraffin-embedded human testicular tissue stained for LZTFL1 using ab236795 at 1/100 dilution in immunohistochemical analysis.
U-251 MG (human brain glioma cell line) cells stained for LZTFL1 (green) using ab236795 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
LZTFL1 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236795 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate.
Lane 2: ab236795 IP in HepG2 whole cell extract.
Lane 3: HepG2 whole cell lysate (20 μg) Input.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236795 has not yet been referenced specifically in any publications.